Ticlopidine inhibits activation of mitogen-activated protein kinase by platelet-derived growth factor in cultured rat renal mesangial cells
Masako Nomiyama; Noriaki Ohnishi; Kazuki Nagasawa; Teruyoshi Yokoyama
Clinical and Experimental Nephrology 2(2): 117-123
ISSN/ISBN: 1342-1751 DOI: 10.1007/bf02479932
Background We previously found that ticlopidine inhibits the proliferation of cultured rat mesangial cells that is induced by fetal bovine serum. This study was designed to examine the effects of ticlopidine on platelet-derived growth factor (PDGF)-induced Dna synthesis and mitogen-activated protein (MAP) kinase activation in such mesangial cells to clarify the mechanism of the antiproliferative action. Methods Glomerular mesangial cells were isolated from rat kidneys, and cells were incubated with various combinations of ticlopidine, PDGF, epidermal growth factor, phorbol 12-acetate 13-myristate (PMA), cilostazol (a phosphodiesterase inhibitor), and H-89 (a c AMP-dependent protein kinase A inhibitor).