Isolation and Characterization of anAGAMOUSHomologuePmAGfrom the Japanese Apricot (Prunus mume Sieb. et Zucc.)

Ji-Hua Hou; Zhi-Hong Gao; Zhen Zhang; Su-Mei Chen; Toshi Ando; Ji-Yu Zhang; Xin-Wei Wang

Plant Molecular Biology Reporter 29(2): 473-480

2011


ISSN/ISBN: 0735-9640
DOI: 10.1007/s11105-010-0248-3
Accession: 064084672

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Abstract
An Agamous homologue, named Pm Ag (Gen Bank accession number EU068730), was isolated from the Japanese apricot cultivar 'Shirokaga' (Prunus mume) via the homology cloning method. The c Dna was 812-base pairs long with an open reading frame of 732 base pairs, and encoded for a putative protein of 243 amino acid residues. Sequence alignment showed that the sequence of Pm Ag was 98% identical to that of the peach (Prunus persica). Genomic Dna analysis revealed an intron just before the stop codon of the ORF, and Pm Ag had Ag motifs I and Ii in its C-terminal region, which suggested that Pm Ag was an Agamous homologue. Real-time quantitative reverse transcription polymerase chain reaction and in situ hybridization showed that the Pm Ag m Rna was highly expressed in the sepals, carpel and stamens, and a weak signal was detected in the seed and nutlet. No expression was detected in the leaves or petals. Overexpression of Pm Ag in transgenic tobacco exhibited no effect on the phenotype of the flower organs, but changes in the floral color from pink to white.