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Feature-preserving noise reduction by using time-domain Gaussian-weighted multiple noise reduction filters for real-time bioluminescence measurement

Feature-preserving noise reduction by using time-domain Gaussian-weighted multiple noise reduction filters for real-time bioluminescence measurement

Analytical Biochemistry 551: 1-3

This paper proposes a time-domain Gaussian-weighted noise reduction filter for bioluminescence measurement with low signal-to-noise ratio through photon counting. The filter was used for estimating the true fold-change signal from noisy gene expression data obtained through real-time dual-color luciferase assay. Furthermore, not only was the higher harmonics noise of the measurement system confirmed to reduce from the gene expression data but rapid and slow changes were also preserved in the estimated signal. In addition, the probability value of Pearson's chi-squared test was improved 257 times at most and 1.5 times on average without impairing the noise reduction ratio.

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Accession: 065149733

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PMID: 29727603

DOI: 10.1016/j.ab.2018.04.026

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