+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom

Functional and proteomic comparison of Bothrops jararaca venom from captive specimens and the Brazilian Bothropic Reference Venom

Journal of Proteomics 174: 36-46

ISSN/ISBN: 1874-3919

PMID: 29275044

DOI: 10.1016/j.jprot.2017.12.008

Snake venom is a variable phenotypic trait, whose plasticity and evolution are critical for effective antivenom production. A significant reduction of the number of snake donations to Butantan Institute (São Paulo, Brazil) occurred in recent years, and this fact may impair the production of the Brazilian Bothropic Reference Venom (BBRV). Nevertheless, in the last decades a high number of Bothrops jararaca specimens have been raised in captivity in the Laboratory of Herpetology of Butantan Institute. Considering these facts, we compared the biochemical and biological profiles of B. jararaca venom from captive specimens and BBRV in order to understand the potential effects of snake captivity upon the venom composition. Electrophoretic analysis and proteomic profiling revealed few differences in venom protein bands and some differentially abundant toxins. Comparison of enzymatic activities showed minor differences between the two venoms. Similar cross-reactivity recognition pattern of both venoms by the antibothropic antivenom produced by Butantan Institute was observed. Lethality and neutralization of lethality for B. jararaca venom from captive specimens and BBRV showed similar values. Considering these results we suggest that the inclusion of B. jararaca venom from captive specimens in the composition of BBRV would not interfere with the quality of this reference venom. Snakebite envenomation is a neglected tropical pathology whose treatment is based on the use of specific antivenoms. Bothrops jararaca is responsible for the majority of snakebites in South and Southeastern Brazil. Its venom shows individual, sexual, and ontogenetic variability, however, the effect of animal captivity upon venom composition is unknown. Considering the reduced number of wild-caught snakes donated to Butantan Institute in the last decades, and the increased life expectancy of the snakes raised in captivity in the Laboratory of Herpetology, this work focused on the comparative profiling of B. jararaca venom from captive snakes and the Brazilian Bothropic Reference Venom (BBRV). BBRV is composed of venom obtained upon the first milking of wild-caught B. jararaca specimens, and used to assess the potency of all bothropic antivenoms produced by Brazilian suppliers. The use of proteomic strategies, added to biochemical and neutralization tests, allowed to conclude that, despite some subtle differences detected between these two venoms, venom from captive specimens could be used in the BBRV composition without affecting its quality in antivenom potency assays.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065163798

Download citation: RISBibTeXText

Related references

Activation of Bothrops jararaca snake venom gland and venom production: a proteomic approach. Journal of Proteomics 94: 460-472, 2013

Assessment of efficacy of bothropic antivenom therapy on microcirculatory effects induced by Bothrops jararaca snake venom. Toxicon: official journal of the International Society on Toxinology 41(5): 583-593, 2003

Proteomic and functional profiling of the venom of Bothrops ayerbei from Cauca, Colombia, reveals striking interspecific variation with Bothrops asper venom. Journal of Proteomics 96: 159-172, 2014

Bothrops fonsecai snake venom activities and cross-reactivity with commercial bothropic venom. Comparative Biochemistry and Physiology. Toxicology and Pharmacology: Cbp 191: 86-100, 2017

Comparison of immunological, biochemical and biophysical properties of three hemorrhagic factors isolated from the venom of Bothrops jararaca (jararaca). Toxicon: Official Journal of the International Society on Toxinology 24(9): 943-946, 1986

Proteomic identification of gender molecular markers in Bothrops jararaca venom. Journal of Proteomics 139: 26-37, 2016

In vitro cleavage of bioactive peptides by peptidases from Bothrops jararaca venom and its neutralization by bothropic antivenom produced by Butantan Institute: Major contribution of serine peptidases. Toxicon: official journal of the International Society on Toxinology 137: 114-119, 2017

Biophysical properties and amino acid composition of Bothrops protease A, a proteolytic enzyme isolated from the venom of the snake Bothrops jararaca (jararaca). Toxicon: Official Journal of the International Society on Toxinology 21(3): 421-427, 1983

Individual variability in the venom proteome of juvenile Bothrops jararaca specimens. Journal of Proteome Research 12(10): 4585-4598, 2013

Alteration of the protein composition of Bothrops jararaca venom and venom gland by isoproterenol treatment. International Journal of Biochemistry 25(10): 1491-1496, 1993

Stimulation of the a-adrenoceptor triggers the venom production cycle in the venom gland of Bothrops jararaca. The Journal of Experimental Biology 207(3): 1-16, 2004

Characterization of -adrenoceptors responsible for venom production in the venom gland of the snake Bothrops jararaca. Life Sciences 67(3): 217-226, 2000

Stimulation of the -adrenoceptor triggers the venom production cycle in the venom gland of Bothrops jararaca. Journal of Experimental Biology uary; 207(3): 411-416, 2004

A nontoxic derivative of Bothrops jararaca venom suitable to generate antibodies against the native venom. Dtw. Deutsche Tierarztliche Wochenschrift 99(4): 143-145, 1992

Immunolocalization of venom metalloproteases in venom glands of adult and of newborn snakes of Bothrops jararaca. Tissue and Cell 34(6): 381-389, 2002