+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of the Novel Nup188-brr7 Allele in a Screen for Cold-Sensitive mRNA Export Mutants in Saccharomyces cerevisiae

Identification of the Novel Nup188-brr7 Allele in a Screen for Cold-Sensitive mRNA Export Mutants in Saccharomyces cerevisiae

G3 8(9): 2991-3003

The maturation and export of mRNA from the nucleus through the nuclear pore complex is critical for maintaining an appropriate proteome in all eukaryotic cells. Here we summarize a previously unpublished screen in S. cerevisiae that utilized an established dT50 in situ hybridization assay to identify cold-sensitive mutants that accumulated bulk poly A RNA in the nucleus. The screen identified seven mutants in six complementation groups, including the brr6-1 strain that we described previously. In addition to brr6-1, we identified novel alleles of the key transport gene GLE1 and NUP188, a component of the Nic96 nucleoporin complex. Notably, we show that the nup188-brr7 allele causes defects in select protein import pathways as well as mRNA export. Given recent structural and functional evidence linking the Nic96 complex to transport components, this mutant may be particularly useful to the transport community.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065213957

Download citation: RISBibTeXText

PMID: 30021831

DOI: 10.1534/g3.118.200447

Related references

A genetic screen in Saccharomyces cerevisiae identifies new genes that interact with mex67-5, a temperature-sensitive allele of the gene encoding the mRNA export receptor. Molecular Genetics and Genomics 281(1): 125-134, 2009

Nuclear protein import, but not mRNA export, is defective in all Saccharomyces cerevisiae mutants that produce temperature-sensitive forms of the Ran GTPase homologue Gsp1p. Molecular & General Genetics. 257(6): 624-634, Il, 1998

The product of the Saccharomyces cerevisiae RSS1 gene, identified as a high-copy suppressor of the rat7-1 temperature-sensitive allele of the RAT7/NUP159 nucleoporin, is required for efficient mRNA export. Molecular Biology of the Cell 7(10): 1601-1621, 1996

Isolation and characterization of Saccharomyces cerevisiae mutants unable to export mRNA from the nucleus. Molecular Biology of the Cell 4(Suppl. ): 420A, 1993

Genome-wide screen for oxalate-sensitive mutants of Saccharomyces cerevisiae. Applied and Environmental Microbiology 73(18): 5919-5927, 2007

Cold sensitive mutants of saccharomyces cerevisiae. Federation Proceedings 29(2): 940, 1970

Photoreactivation of saccharomyces cerevisiae cells after irradiation with 25 mev electrons the influence of the photoreactivable damage on the oxygen effect manifestation in radio sensitive and uv sensitive saccharomyces cerevisiae mutants. Radiobiologiya 26(4): 460-464, 1986

Identification of constitutively active AtMPK6 mutants using a functional screen in Saccharomyces cerevisiae. Methods in Molecular Biology 1171: 67-77, 2014

Isolation of temperature-sensitive mutants for mRNA capping enzyme in Saccharomyces cerevisiae. Molecular & General Genetics. 249(2): 147-154, 1995

Isolation of temperature-sensitive mutants mRNA capping enzyme in Saccharomyces cerevisiae. Molecular and general genetics: MGG 249(2): 147-154, 1995

Cold sensitive cell division cycle mutants of yeast saccharomyces cerevisiae isolation properties and pseudoreversion studies. Genetics 100(4): 547-564, 1982

The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae. Embo Journal 18(13): 3746-3756, 1999

Photoreactivation in Saccharomyces cerevisiae cells after irradiation with electrons (25 MeV). The role of photoreactivated damage in the manifestation of oxygen effects in radio- and UV-sensitive mutants of Saccharomyces cerevisiae. Radiobiologiia 26(4): 460-464, 1986

A genetic screen for increasing metabolic flux in the isoprenoid pathway of Saccharomyces cerevisiae : Isolation of SPT15 mutants using the screen. Metabolic Engineering Communications 3: 164-172, 2016

Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31. Dna Repair 5(12): 1459-1474, 2006