+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Intranasal monkeypox marmoset model: Prophylactic antibody treatment provides benefit against severe monkeypox virus disease

Intranasal monkeypox marmoset model: Prophylactic antibody treatment provides benefit against severe monkeypox virus disease

Plos Neglected Tropical Diseases 12(6): E0006581

ISSN/ISBN: 1935-2727

PMID: 29927927

DOI: 10.1371/journal.pntd.0006581

Concerns regarding outbreaks of human monkeypox or the potential reintroduction of smallpox into an immunological naïve population have prompted the development of animal models and countermeasures. Here we present a marmoset model of monkeypox and smallpox disease utilizing a relevant poxvirus via a natural exposure route. We found that 1000 plaque forming units (PFU) of Monkeypox virus was sufficient to recapitulate smallpox disease, to include an incubation period of approximately 13 days, followed by the onset of rash, and death between 15 and 17 days. Temporally accurate manifestation of viremia and oral shedding were also features. The number of lesions ranged from no lesions to 299, the most reported in a marmoset exposed to a poxvirus. To both evaluate the efficacy of our antibodies and the applicability of the model system, marmosets were prophylactically treated with two monoclonal antibodies, c7D11 and c8A. Of three marmosets, two were completely free of disease and a single marmoset died 8 days after the mock (n = 1) or PBS control(s) (n = 2). Evaluation of the serum levels of the three animals provided a possible explanation to the animal succumbing to disease. Interestingly, more females had lesions (and a greater number of lesions) and lower viral burden (viremia and oral shedding) than males in our studies, suggesting a possible gender effect.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065253379

Download citation: RISBibTeXText

Related references

Susceptibility of Marmosets (Callithrix jacchus) to Monkeypox Virus: a Low Dose Prospective Model for Monkeypox and Smallpox Disease. Plos one 10(7): E0131742, 2015

Deletion of the monkeypox virus inhibitor of complement enzymes locus impacts the adaptive immune response to monkeypox virus in a nonhuman primate model of infection. Journal of Virology 85(18): 9527-9542, 2011

Development of the disease in marmot at the intranasal infection with the monkeypox virus. Voprosy Virusologii 60(6): 37-41, 2015

Transmissibility of the monkeypox virus clades via respiratory transmission: investigation using the prairie dog-monkeypox virus challenge system. Plos one 8(2): E55488, 2013

Infection-competent monkeypox virus contamination identified in domestic settings following an imported case of monkeypox into the UK. Environmental Microbiology 2022, 2022

Comparison of white pock (h) mutants of monkeypox virus with parental monkeypox and with variola-like viruses isolated from animals. Nature 286(5768): 29-32, 1980

Systems kinomics demonstrates Congo Basin monkeypox virus infection selectively modulates host cell signaling responses as compared to West African monkeypox virus. Molecular and Cellular Proteomics: Mcp 11(6): M111.015701, 2012

Pathogenesis of fulminant monkeypox with bacterial sepsis after experimental infection with West African monkeypox virus in a cynomolgus monkey. International Journal of Clinical and Experimental Pathology 7(7): 4359-4370, 2014

IMVAMUNE® and ACAM2000® Provide Different Protection against Disease when Administered Postexposure in an Intranasal Monkeypox Challenge Prairie Dog Model. Vaccines 8(3), 2020

Application of the Ibis-T5000 pan-Orthopoxvirus assay to quantitatively detect monkeypox viral loads in clinical specimens from macaques experimentally infected with aerosolized monkeypox virus. American Journal of Tropical Medicine and Hygiene 82(2): 318-323, 2010

Variole ou varicelle grave ? un cas de variole humaine à monkeypox virus chez un enfant camerounais - Smallpox or Chickenpox? A case of human Smallpox due to Monkeypox Virus in a child in Cameroun. Annales de la Societe Belge de Medecine Tropicale 71(2): 123-128, 1991

A case of severe monkeypox virus disease in an American child: emerging infections and changing professional values. Pediatric Infectious Disease Journal 22(12): 1093-6; Discussion 1096-8, 2003

Do Monkeypox Exposures Vary by Ethnicity? Comparison of Aka and Bantu Suspected Monkeypox Cases. American Journal of Tropical Medicine and Hygiene 102(1): 202-205, 2020

Re-emergence of human monkeypox in Zaire in 1996. Monkeypox Epidemiologic Working Group. Lancet 349(9063): 1449-1450, 1997

Effective antiviral treatment of systemic orthopoxvirus disease: ST-246 treatment of prairie dogs infected with monkeypox virus. Journal of Virology 85(17): 9176-9187, 2011