+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Mechanisms for rescue of corpus luteum during pregnancy: gene expression in bovine corpus luteum following intrauterine pulses of prostaglandins E1 and F2α

Mechanisms for rescue of corpus luteum during pregnancy: gene expression in bovine corpus luteum following intrauterine pulses of prostaglandins E1 and F2α

Biology of Reproduction 98(4): 465-479

In ruminants, uterine pulses of prostaglandin (PG) F2α characterize luteolysis, while increased PGE2/PGE1 distinguish early pregnancy. This study evaluated intrauterine (IU) infusions of PGF2α and PGE1 pulses on corpus luteum (CL) function and gene expression. Cows on day 10 of estrous cycle received 4 IU infusions (every 6 h; n = 5/treatment) of saline, PGE1 (2 mg PGE1), PGF2α (0.25 mg PGF2α), or PGE1 + PGF2α. A luteal biopsy was collected at 30 min after third infusion for determination of gene expression by RNA-Seq. As expected, IU pulses of PGF2α decreased (P < 0.01) P4 luteal volume. However, there were no differences in circulating P4 or luteal volume between saline, PGE1, and PGE1 + PGF2α, indicating inhibition of PGF2α-induced luteolysis by IU pulses of PGE1. After third pulse of PGF2α, luteal expression of 955 genes were altered (false discovery rate [FDR] < 0.01), representing both typical and novel luteolytic transcriptomic changes. Surprisingly, after third pulse of PGE1 or PGE1 + PGF2α, there were no significant changes in luteal gene expression (FDR > 0.10) compared to saline cows. Increased circulating concentrations of the metabolite of PGF2α (PGFM; after PGF2α and PGE1 + PGF2α) and the metabolite PGE (PGEM; after PGE1 and PGE1 + PGF2α) demonstrated that PGF2α and PGE1 are entering bloodstream after IU infusions. Thus, IU pulses of PGF2α and PGE1 allow determination of changes in luteal gene expression that could be relevant to understanding luteolysis and pregnancy. Unexpectedly, by third pulse of PGE1, there is complete blockade of either PGF2α transport to the CL or PGF2α action by PGE1 resulting in complete inhibition of transcriptomic changes following IU PGF2α pulses.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065305192

Download citation: RISBibTeXText

PMID: 29293919

DOI: 10.1093/biolre/iox183

Related references

Differences of microvascular endothelium in the bovine corpus luteum of pregnancy and the corpus luteum of the estrous cycle. Biology of the Cell 87(3): 179-188, 1996

Angiogenesis in the human corpus luteum: changes in expression of angiopoietins in the corpus luteum throughout the menstrual cycle and in early pregnancy. Journal of Clinical Endocrinology and Metabolism 90(11): 6141-6148, 2005

Variations in ovulatory response associated with the use of prostaglandins to manipulate the lifespan of the normal diestrous corpus luteum or the prolonged corpus luteum of the mare. Proceedings of the annual convention American Association of Equine Practitioners: ub 1979) (24th) 173-175, 1979

Anatomy and physiology of the corpus luteum of cattle during pregnancy and after parturition, with special reference to the pressing out of the corpus luteum of pregnancy in cases of retention of the after-birth. Dissertation, tierarztl. Hochsch, Berlin, 30, 1934

Repeated intrauterine infusions of lipopolysaccharide alter gene expression and lifespan of the bovine corpus luteum. Journal of Dairy Science 99(8): 6639-6653, 2016

Differential regulation of apoptosis in the corpus luteum of pregnancy and newly formed corpus luteum after parturition in rats. Biology of Reproduction 70(2): 313-318, 2003

Ehrhardt, K.: Clinical Experiments on Pregnancy Maintaining Effect of Corpus Luteum Hormone After Loss of Corpus Luteum of Pregnancy. American Journal of Obstetrics and Gynecology 44(5): 917-918, 1942

Biosynthesis of steroids in the corpus luteum. (General review of literature and radioisotope study related to menstrual and pregnancy corpus luteum in humans. Annali di Ostetricia Ginecologia Medicina Perinatale 92(11): 772-794, 1971

Physiology of corpus luteum VII Maintenance of pregnancy in rabbit after very early castration, by corpus luteum extracts. Proc Soc Exp Biol & Med: 403-405, 1930

Patterns of gene expression in the bovine corpus luteum following repeated intrauterine infusions of low doses of prostaglandin F2alpha. Biology of Reproduction 86(4): 130, 2012

Hemoperitoneum from rupture of the corpus luteum; report of a case of ruptured corpus luteum of pregnancy. Pennsylvania Medical Journal 50(11): 1161-1163, 1947

Expression of melatonin and its related synthase and membrane receptors in the oestrous corpus luteum and corpus luteum verum of sheep. Reproduction in Domestic Animals 53(5): 1142-1148, 2018

The functions of the corpus luteum. V. Changes in the sterile horn during pregnancy and their relation to changes in the corpus luteum. Proceedings of the Royal Society London, 109B 196-213, 1931

The Functions of the Corpus Luteum. V.--Changes in the Sterile Horn During Pregnancy, and their Relation to Changes in the Corpus Luteum. Proceedings Biological Sciences 109(761): 196-213, 1931

Expression of LGR7 in the primate corpus luteum implicates the corpus luteum as a relaxin target organ. Annals of the New York Academy of Sciences 1160: 147-151, 2009