+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis



Phylogenomic analysis of the species of the Mycobacterium tuberculosis complex demonstrates that Mycobacterium africanum, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii are later heterotypic synonyms of Mycobacterium tuberculosis



International Journal of Systematic and Evolutionary Microbiology 68(1): 324-332



The species within the Mycobacterium tuberculosis Complex (MTBC) have undergone numerous taxonomic and nomenclatural changes, leaving the true structure of the MTBC in doubt. We used next-generation sequencing (NGS), digital DNA-DNA hybridization (dDDH), and average nucleotide identity (ANI) to investigate the relationship between these species. The type strains of Mycobacterium africanum, Mycobacterium bovis, Mycobacterium caprae, Mycobacterium microti and Mycobacterium pinnipedii were sequenced via NGS. Pairwise dDDH and ANI comparisons between these, previously sequenced MTBC type strain genomes (including 'Mycobacterium canettii', 'Mycobacterium mungi' and 'Mycobacterium orygis') and M. tuberculosis H37RvT were performed. Further, all available genome sequences in GenBank for species in or putatively in the MTBC were compared to H37RvT. Pairwise results indicated that all of the type strains of the species are extremely closely related to each other (dDDH: 91.2-99.2 %, ANI: 99.21-99.92 %), greatly exceeding the respective species delineation thresholds, thus indicating that they belong to the same species. Results from the GenBank genomes indicate that all the strains examined are within the circumscription of H37RvT (dDDH: 83.5-100 %). We, therefore, formally propose a union of the species of the MTBC as M. tuberculosis. M. africanum, M. bovis, M. caprae, M. microti and M. pinnipedii are reclassified as later heterotypic synonyms of M. tuberculosis. 'M. canettii', 'M. mungi', and 'M. orygis' are classified as strains of the species M. tuberculosis. We further recommend use of the infrasubspecific term 'variant' ('var.') and infrasubspecific designations that generally retain the historical nomenclature associated with the groups or otherwise convey such characteristics, e.g. M. tuberculosis var. bovis.

(PDF emailed within 1 workday: $29.90)

Accession: 065406702

Download citation: RISBibTeXText

PMID: 29205127


Related references

In vitro susceptibility of Mycobacterium tuberculosis, Mycobacterium africanum, Mycobacterium bovis, Mycobacterium avium, Mycobacterium fortuitum, and Mycobacterium chelonae to ticarcillin in combination with clavulanic acid. Antimicrobial Agents and ChemoTherapy 31(1): 132-133, 1987

Dna relatedness among selected strains of mycobacterium tuberculosis mycobacterium bovis mycobacterium bovis bcg mycobacterium microti and mycobacterium africanum. International Journal of Systematic Bacteriology 35(2): 147-150, 1985

Infections with mycobacterium avium human mycobacterium bovis mycobacterium tuberculosis mycobacterium kansasii mycobacterium intracellulare mycobacterium xenopei. Lancet (7558): 36, 1968

Monoclonal antibodies for the rapid differentiation of mycobacterium tuberculosis from mycobacterium bovis mycobacterium avium mycobacterium kansasii and mycobacterium xenopi. Abstracts of the Annual Meeting of the American Society for Microbiology 88: 139, 1988

In vitro activities of norfloxacin and ciprofloxacin against mycobacterium tuberculosis mycobacterium avium complex mycobacterium chelonei mycobacterium fortuitum and mycobacterium kansasii. Antimicrobial Agents & Chemotherapy 26(1): 94-96, 1984

Delayed type skin reactions in guinea pigs sensitized with various acid fast bacilli and tested with protoplasms from homologous and heterologous organisms mycobacterium tuberculosis mycobacterium balnei mycobacterium batteyi mycobacterium bovis mycobacterium kansasii. International Archives Of Allergy & Applied Immunology: 283-292, 1968

In vitro activity of ciprofloxacin and ofloxacin against mycobacterium tuberculosis mycobacterium avium mycobacterium africanum mycobacterium kansasii and strains of bcg. Immunitaet und Infektion 14(6): 203-207, 1986

Genes for immunodominant protein antigens are highly homologous in Mycobacterium tuberculosis, Mycobacterium africanum, and the vaccine strain Mycobacterium bovis BCG. Infection and Immunity 55(10): 2378-2382, 1987

Biochemical and genetic evidence for the transfer of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to the species Mycobacterium bovis Karlson and Lessel 1970 (approved lists 1980) as Mycobacterium bovis subsp. caprae comb. nov. International Journal of Systematic and Evolutionary Microbiology 52(Pt 2): 433-436, 2002

Further studies on african strains of mycobacterium tuberculosis comparison with mycobacterium bovis and mycobacterium microti. Annales des Societes Belges de Medecine Tropicale de Parasitologie et de Mycologie 50(2): 211-228, 1970

Comparative study of immunizing and delayed hypersensitivity eliciting antigens of mycobacterium leprae mycobacterium tuberculosis mycobacterium vaccae and mycobacterium bovis bcg. International Journal of Leprosy & Other Mycobacterial Diseases 55(1): 42-53, 1987

Periodic acid-Schiff reaction stains Mycobacterium tuberculosis, Mycobacterium leprae, Mycobacterium ulcerans, Mycobacterium chelonei (abscessus), and Mycobacterium kansasii. Archives of Pathology and Laboratory Medicine 109(8): 701-703, 1985

Electron cytochemical study of cytochrome oxidase and peroxidase of mycobacterium leprae mycobacterium lepraemurium mycobacterium tuberculosis mycobacterium kansasii and mycobacterium balnei. International Journal Of Leprosy & Other Mycobacterial Diseasessuppl: 360-361, 1979

Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates. International Journal of Systematic and Evolutionary Microbiology 54(Pt 5): 1653-1667, 2004

Activity of rifapentine and its metabolite 25-O-desacetylrifapentine compared with rifampicin and rifabutin against Mycobacterium tuberculosis, Mycobacterium africanum, Mycobacterium bovis and M. bovis BCG. Journal of Antimicrobial ChemoTherapy 46(4): 565-570, 2000