+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates



Temperature, pH and Trimethoprim-Sulfamethoxazole Are Potent Inhibitors of Biofilm Formation by Stenotrophomonas maltophilia Clinical Isolates



Polish Journal of Microbiology 66(4): 433-438



Stenotrophomonas maltophilia, an opportunistic pathogen usually connected with healthcare-associated infections, is an environmental bacterium. Intrinsic resistance to multiple antibiotics, with different virulence determinants in the last decade classified this bacterium in the group of global multiple drug resistant (MDR) organism. S. maltophilia clinical isolates, were collected from tertiary care pediatric hospital in Belgrade, Serbia to investigate influence of different factors on biofilm formation, kinetics of biofilm formation for strong biofilm producers and effect of trimethoprim-sulfamethoxazole (TMP/SMX) on formed biofilm. Most of the isolates (89.8%) were able to form a biofilm. Analysis of biofilm formation in different growth conditions showed that changing of temeperature and pH had the stronggest effect on biofilm formation almost equally in group of cystic fibrosis (CF) and non-CF strains. TMP/SMX in concentration of 50 μg/ml reduced completely 24 h old biofilms while concentration of 25 μg/ml effects formed biofilms in a strain dependent manner. Among strains able to form strong biofilm CF isolates formed biofilm slower than non-CF isolates, while shaking conditions did not affect biofilm formation. Swimming motility was detected in both CF and non-CF isolates, however more motile strain formed stronger biofilms. This study suggests that temperature, pH and TMP/SMX had the strongest influence on biofilm formation in analyzed collection of S. maltophilia. A positive correlation between motility and strength of formed biofilm was demonstrated.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 065562310

Download citation: RISBibTeXText

PMID: 29319523


Related references

Biofilm Formation by Stenotrophomonas maltophilia: Modulation by Quinolones, Trimethoprim-Sulfamethoxazole, and Ceftazidime. Antimicrobial Agents and Chemotherapy 48(1): 151-160, 2004

Biofilm formation by Stenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime. Antimicrobial Agents and ChemoTherapy 48(1): 151-160, 2004

In vitro killing effect of moxifloxacin on clinical isolates of Stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole. Antimicrobial Agents and ChemoTherapy 46(12): 3997-3999, 2002

Increased incidence of class 1 integrons in trimethoprim/sulfamethoxazole-resistant clinical isolates of Stenotrophomonas maltophilia. Journal of Antimicrobial ChemoTherapy 59(5): 1038-1039, 2007

High incidence of class 1 integron in trimethoprim-sulfamethoxazole resistant clinical isolates of Stenotrophomonas maltophilia. 2007

Increase in the Prevalence of Resistance Determinants to Trimethoprim/Sulfamethoxazole in Clinical Stenotrophomonas maltophilia Isolates in China. Plos one 11(6): E0157693, 2016

In vitro antibacterial and antibiofilm activities of chlorogenic acid against clinical isolates of Stenotrophomonas maltophilia including the trimethoprim/sulfamethoxazole resistant strain. Biomed Research International 2013: 392058, 2013

Comparisons between patients with trimethoprim-sulfamethoxazole-susceptible and trimethoprim-sulfamethoxazole-resistant Stenotrophomonas maltophilia monomicrobial bacteremia: A 10-year retrospective study. Journal of Microbiology Immunology and Infection 49(3): 378-386, 2016

Role of sul2 gene linked to transposase in resistance to trimethoprim/sulfamethoxazole among Stenotrophomonas maltophilia isolates. Annals of Laboratory Medicine 36(1): 73-75, 2016

Class 1 integrons increase trimethoprim-sulfamethoxazole MICs against epidemiologically unrelated Stenotrophomonas maltophilia isolates. Antimicrobial Agents and ChemoTherapy 48(2): 666-669, 2004

Study of class I integrons from epidemiologically unrelated Stenotrophomonas maltophilia isolates and the increase of trimethoprim-sulfamethoxazole resistance. Abstracts of the General Meeting of the American Society for Microbiology 103: A-052, 2003

Effect of environmental factors on biofilm formation by clinical Stenotrophomonas maltophilia isolates. Folia Microbiologica 52(1): 86-90, 2007

Clinical implications of trimethoprim-sulfamethoxazole -resistant Stenotrophomonas maltophilia. Abstracts of the Interscience Conference on Antimicrobial Agents & Chemotherapy 39: 618, 1999

Clinical implications of stenotrophomonas maltophilia resistant to trimethoprim-sulfamethoxazole: a study of 69 patients at 2 university hospitals. Scandinavian Journal of Infectious Diseases 32(6): 651-656, 2000

Clinical Implications of Stenotrophomonas maltophilia Resistant to Trimethoprim-Sulfamethoxazole: a Study of 69 Patients at 2 University Hospitals. Scandinavian Journal of Infectious Diseases 32(6): 651-656, 2000