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The application of conventional smears and liquid-based cytologic slides of lymphatic metastasis specimens of lung adenocarcinoma acquired by fine needle aspiration to detect the expression of anaplastic lymphoma kinase protein



The application of conventional smears and liquid-based cytologic slides of lymphatic metastasis specimens of lung adenocarcinoma acquired by fine needle aspiration to detect the expression of anaplastic lymphoma kinase protein



Zhonghua Zhong Liu Za Zhi 40(6): 422-427



目的: 探讨细针吸取细胞学传统涂片和液基薄片标本应用免疫细胞化学(ICC)检测肺腺癌淋巴结转移灶间变性淋巴瘤激酶(ALK,D5F3)蛋白表达的可行性。 方法: 收集147例进展期肺腺癌淋巴结转移灶的细针吸取细胞学标本,其中传统涂片47例,液基薄片100例,采用ICC法检测ALK蛋白的表达,并与逆转录聚合酶链反应(RT-PCR)、荧光原位杂交(FISH)检测结果进行比较。 结果: ALK蛋白在肺腺癌淋巴结转移灶细针吸取细胞学标本中的阳性率为11.6%(17/147),其中在传统涂片和液基薄片中的阳性率分别为10.6%(5/47)和12.0%(12/100)。147例标本中,有57例标本采用RT-PCR或FISH方法进行验证,其中阳性17例,阴性40例,总符合率为96.5%(55/57)。肺腺癌淋巴结转移灶细针吸取细胞学标本检测ALK蛋白的灵敏度为94.1%(16/17),特异度为97.5%(39/40);其中传统涂片检测ALK蛋白的灵敏度为100%(5/5),特异度为100%(10/10);液基薄片检测ALK蛋白的灵敏度和特异度分别为91.7%(11/12)和96.7%(29/30)。 结论: 肺腺癌患者无法获取手术标本或标本无法制成细胞块时,细针吸取细胞学传统涂片和液基薄片可以用来进行ALK蛋白的检测。.

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Accession: 065567044

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PMID: 29936767


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