+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Energy Landscape of Negatively Charged BSA Adsorbed on a Negatively Charged Silica Surface

Energy Landscape of Negatively Charged BSA Adsorbed on a Negatively Charged Silica Surface

Journal of Physical Chemistry. B 122(14): 3744-3753

We study the energy landscape of the negatively charged protein bovine serum albumin adsorbed on a negatively charged silica surface at pH 7. We use fully atomistic molecular dynamics (MD) and steered MD (SMD) to probe the energy of adsorption and the pathway for the surface diffusion of the protein and its associated activation energy. We find an adsorption energy ∼1.2 eV, which implies that adsorption is irreversible even on experimental time scales of hours. In contrast, the activation energy for surface diffusion is ∼0.4 eV so that it is observable on the MD simulation time scale of 100 ns. This analysis paves the way for a more detailed understanding of how a protein layer forms on biomaterial surfaces, even when the protein and surface share the same electrical polarity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065583069

Download citation: RISBibTeXText

PMID: 29536734

DOI: 10.1021/acs.jpcb.7b12484

Related references

How Negatively Charged Proteins Adsorb to Negatively Charged Surfaces: A Molecular Dynamics Study of BSA Adsorption on Silica. Journal of Physical Chemistry. B 120(40): 10463-10468, 2016

The effect of adsorbed charged polypeptides on the electrophoretic mobility of positively and negatively charged polystyrene latices. Journal of Colloid & Interface Science 118(2): 366-378, 1987

On the growth-stunting effect of negatively charged air-ionization on livestock: A contribution to the problem of the germ-inhibiting action of negatively-charged air ions. 1972

Effect of the electrostatic interaction on the redox reaction of positively charged cytochrome C adsorbed on the negatively charged surfaces of acid-terminated alkanethiol monolayers on a Au(111) electrode. Langmuir 21(4): 1470-1474, 2005

Carbon nanoparticle surface functionalisation: converting negatively charged sulfonate to positively charged sulfonamide. Physical Chemistry Chemical Physics 12(18): 4872-4878, 2010

Coarse-grained modeling of proline rich protein 1 (PRP-1) in bulk solution and adsorbed to a negatively charged surface. Journal of Physical Chemistry. B 110(24): 12141-8, 2006

Lysozyme and bovine serum albumin adsorption on uncoated silica and AlOOH-coated silica particles: the influence of positively and negatively charged oxide surface coatings. Biomaterials 26(21): 4351-4357, 2005

Regioselective hydrolysis of human serum albumin by Zr(IV)-substituted polyoxotungstates at the interface of positively charged protein surface patches and negatively charged amino acid residues. Chemistry 20(14): 3894-3897, 2014

The surface potential of insulating thin films negatively charged by a low-energy focused electron beam. Micron 41(5): 416-422, 2010

Adsorbed proteins mask negatively charged sites of the erythrocyte glycocalyx. Acta Histochemica 60(2): 312-316, 1977

Electrokinetic behavior of F(ab)2 fragments adsorbed onto positively and negatively charged polymer colloids. Colloids and Surfaces A: Physicochemical and Engineering Aspects 95(2-3): 261-269, 1995

Electrostatic repulsion of positively charged vesicles and negatively charged objects. Science 285(5426): 4-7, 1999

Interaction between equally charged membrane surfaces mediated by positively and negatively charged macro-ions. Journal of Membrane Biology 236(1): 43-53, 2010

Spectroscopic studies of nanostructures of negatively charged free base porphyrin and positively charged tin porphyrins. Polyhedron 29(5): 1469-1474, 2010

Charged collagen structure mediates the recognition of negatively charged macromolecules by macrophage scavenger receptors. Journal of Biological Chemistry 268(3): 2126-2133, 1993