+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

TUBP1 protein lead to mitochondria-mediated apoptotic cell death in Verticillium dahliae

TUBP1 protein lead to mitochondria-mediated apoptotic cell death in Verticillium dahliae

International Journal of Biochemistry and Cell Biology 103: 35-44

Verticillium wilt, caused by Verticillium dahliae, is a cancer of cotton which affects cotton quality and yield in China. In our previous study, a novel anti-Verticillium dahliae protein TUBP1 was obtained from Bacillus axarquiensis. Then, we have systematically studied the anti-V. dahliae activity and the pore formation action of TUBP1 protein on V. dahliae membrane. In present study, we provide detailed whether TUBP1 protein induced mitochondrial damaged and mitochondria-mediated apoptotic cell death in V. dahliae. In V. dahliae cells exposed to the TUBP1 protein, the mitochondrial dehydrogenases, F0F1-ATPase, malate dehydrogenase (MDH), and succinate dehydrogenase (SDH) activities were reduced and reactive oxygen species (ROS), which is a major cause of apoptosis, were increased. The results demonstrated that mitochondria dysfunction and ROS-induced oxidative stress caused the release of apoptotic factors. The following cellular changes, which are characteristic of apoptosis, were measured including mitochondrial membrane potential (MMP), Cytochrome c (Cyt C) release, metacaspase activation, phosphatidylserine (PS) exposure, and DNA condensation and fragmentation. The results showed that an important feature of apoptosis, MMP, was caused by ROS. Significantly, cyt c was released, which is a factor in metacaspase activity after treatment with the TUBP1 protein. Number of stained cells with activated intracellular metacaspases exposed to TUBP1 protein was increased in a concentration-dependent manner. We also showed that in the early and late stages of apoptosis, the effects of the TUBP1 protein were mediated by PS and DNA fragmentation and condensation in the plasma membrane, respectively. There turned out that the TUBP1 protein led to mitochondria-mediated apoptotic cell death in V.dahliae. The results of this investigation indicated that TUBP1 stain or protein is a potent candidate against V.dahliae infections in crop species.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065642897

Download citation: RISBibTeXText

PMID: 30081099

DOI: 10.1016/j.biocel.2018.08.001

Related references

Isolation and anti-Verticillium dahliae activity from Bacillus axarquiensis TUBP1 protein. Process Biochemistry 51(10): 1691-1698, 2016

Zebrafish anti-apoptotic protein zfBcl-xL can block betanodavirus protein alpha-induced mitochondria-mediated secondary necrosis cell death. Fish and Shellfish Immunology 24(4): 436-449, 2008

An Asparagine-Rich Protein Nbnrp1 Modulate Verticillium dahliae Protein PevD1-Induced Cell Death and Disease Resistance in Nicotiana benthamian a. Frontiers in Plant Science 9: 303, 2018

Modulating Mitochondria-Mediated Apoptotic Cell Death through Targeting of Bcl-2 Family Proteins. Recent Patents on Dna & Gene Sequences 1(1): 43-61, 2007

Modulating mitochondria-mediated apoptotic cell death through targeting of Bcl-2 family proteins. Recent Patents on Dna and Gene Sequences 1(1): 43-61, 2007

Mitochondria mediated cell death in neural stem cells exposed to apoptotic stimuli. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 621 11, 2002

Relationship between dna content and spore volume in sixteen isolates of verticillium lecanii and two new diploids of verticillium dahliae equals verticillium dahliae var longisporum. Journal of General Microbiology 131(12): 3229-3236, 1985

Genetics of verticillium dahliae the causal agent of verticillium wilt of cotton 5. genetic analysis of verticillium dahliae melanin deficient mutants. Genetika 19(2): 284-287, 1983

Genetics of verticillium dahliae the causal agent of verticillium wilt of cotton 6. mutation and recombination analysis of verticillium dahliae micro sclerotia morphogenesis. Genetika 19(2): 288-294, 1983

Apoptotic potential of Fas-associated death domain on regulation of cell death regulatory protein cFLIP and death receptor mediated apoptosis in HEK 293T cells. Journal of Cell Communication and Signaling 6(3): 155-168, 2012

Genetics of verticillium dahliae agent of verticillium wilt of cotton 4. complementation analysis of melanin deficient mutants of verticillium dahliae. Genetika 18(12): 2013-2018, 1982

(+)-Medioresinol leads to intracellular ROS accumulation and mitochondria-mediated apoptotic cell death in Candida albicans. Biochimie 94(8): 1784-1793, 2012

Role of the mitochondria in immune-mediated apoptotic death of the human pancreatic β cell line βLox5. Plos one 6(6): E20617, 2011

The role of mitochondria in cell death Regulation of the pro-apoptotic protein Bak. Biochimica et Biophysica Acta (Bba) - Bioenergetics 1817: S160, 2012

Cathepsin B contributes to traumatic brain injury-induced cell death through a mitochondria-mediated apoptotic pathway. Journal of Neuroscience Research 88(13): 2847-2858, 2010