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Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids



Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids



Methods in Molecular Biology 1728: 67-93



Tub-tag labeling is a chemoenzymatic method that enables the site-specific labeling of proteins. Here, the natural enzyme tubulin tyrosine ligase incorporates noncanonical tyrosine derivatives to the terminal carboxylic acid of proteins containing a 14-amino acid recognition sequence called Tub-tag. The tyrosine derivative carries a unique chemical reporter allowing for a subsequent bioorthogonal modification of proteins with a great variety of probes. Here, we describe the Tub-tag protein modification protocol in detail and explain its utilization to generate labeled proteins for advanced applications in cell biology, imaging, and diagnostics.

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Accession: 065642898

Download citation: RISBibTeXText

PMID: 29404991

DOI: 10.1007/978-1-4939-7574-7_4


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