+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Tuning Length Scales of Small Domains in Cell-Derived Membranes and Synthetic Model Membranes

Tuning Length Scales of Small Domains in Cell-Derived Membranes and Synthetic Model Membranes

Biophysical Journal 115(4): 690-701

Micron-scale, coexisting liquid-ordered (Lo) and liquid-disordered (Ld) phases are straightforward to observe in giant unilamellar vesicles (GUVs) composed of ternary lipid mixtures. Experimentally, uniform membranes undergo demixing when temperature is decreased: domains subsequently nucleate, diffuse, collide, and coalesce until only one domain of each phase remains. The sizes of these two domains are limited only by the size of the system. Under different conditions, vesicles exhibit smaller-scale domains of fixed sizes, leading to the question of what sets the length scale. In membranes with excess area, small domains are expected when coarsening is hindered or when a microemulsion or modulated phase arises. Here, we test predictions of how the size, morphology, and fluorescence levels of small domains vary with the membrane's temperature, tension, and composition. Using GUVs and cell-derived giant plasma membrane vesicles, we find that 1) the characteristic size of domains decreases when temperature is increased or membrane tension is decreased, 2) stripes are favored over circular domains for lipid compositions with low energy per unit interface, 3) fluorescence levels are consistent with domain registration across both monolayer leaflets of the bilayer, and 4) small domains form in GUVs composed of lipids both with and without ester-linked lipids. Our experimental results are consistent with several elements of current theories for microemulsions and modulated phases and inconsistent with others, suggesting a motivation to modify or enhance current theories.

(PDF emailed within 0-6 h: $19.90)

Accession: 065643859

Download citation: RISBibTeXText

PMID: 30049406

DOI: 10.1016/j.bpj.2018.06.027

Related references

n-Alcohol Length Governs Shift in L o -L d Mixing Temperatures in Synthetic and Cell-Derived Membranes. Biophysical Journal 113(6): 1200-1211, 2017

Sonoporation at Small and Large Length Scales: Effect of Cavitation Bubble Collapse on Membranes. Journal of Physical Chemistry Letters 6(3): 413-418, 2016

Seeing the Forest in Lieu of the Trees: Continuum Simulations of Cell Membranes at Large Length Scales. Annual Reports in Computational Chemistry 10: 47-76, 2014

Detection of cholesterol bilayer domains in intact biological membranes: Methodology development and its application to studies of eye lens fiber cell plasma membranes. Experimental Eye Research 178: 72-81, 2018

Transport of alkyl homologs across synthetic and biological membranes: a new model for chain length-activity relationships. Journal of Pharmaceutical Sciences 62(2): 210-217, 1973

Drug permeation through synthetic lipid membranes. 5. Permeability of synthetic lecithin membranes for a group of structurally similar heterocyclics. Die Pharmazie 29(8): 542-543, 1974

The fluid mosaic model of the structure of cell membranes cell membranes are viewed as 2 dimensional solutions of oriented globular proteins and lipids. Science (Washington D C) 175(4023): 720-731, 1972

Detection of lipid domains in model and cell membranes by fluorescence lifetime imaging microscopy. Biochimica et Biophysica Acta 1798(7): 1444-1456, 2010

Supported Membranes Meet Flat Fluidics: Monitoring Dynamic Cell Adhesion on Pump-Free Microfluidics Chips Functionalized with Supported Membranes Displaying Mannose Domains. Materials 6(2): 669-681, 2013

Facilitated phosphatidylserine flip-flop across vesicle and cell membranes using urea-derived synthetic translocases. Organic and Biomolecular Chemistry 2(2): 214-219, 2004

Drug permeability through artificial lipid membranes. 15: Effect of anions on the transport of buformin in model membranes and isolated, lumen- and vessel-perfused small intestine. Die Pharmazie 38(1): 46-47, 1983

Peptides derived from BH3 domains of Bcl-2 family membranes: a comparative analysis of inhibition of Bcl-2, Bcl-xL and Bax oligomerization, induction of cytochrome c release, and activation of cell death. Biochemistry (American Chemical Society) 41(30): 85-95, 2002

Development of fluorophore dynamics imaging as a probe for lipid domains in model vesicles and cell membranes. European Biophysics Journal 40(2): 131-141, 2011

Nanoparticles meet cell membranes: probing nonspecific interactions using model membranes. Environmental Science and Technology 48(2): 873-880, 2014

Proton conduction in exchange membranes across multiple length scales. Accounts of Chemical Research 45(11): 2002-2010, 2012