+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes

Anti-adipogenic effect of flavonoids from Chromolaena odorata leaves in 3T3-L1 adipocytes

Journal of Integrative Medicine 16(6): 427-434

The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new application for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3T3-L1 preadipocytes/adipocytes as a model. Three leaf extracts and thirteen flavonoids isolated from C. odorata were tested for their effect on lipid accumulation in 3T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive control. The effects of active flavonoids on the adipocytes were confirmed by oil red O staining and visualized under a light microscope. n-Hexane and ethyl acetate extracts of C. odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 µg/mL. Four flavonoids, pectolinarigenin, kaempferide, 4,2'-dihydroxy-4',5',6'-trimethoxychalcone and dillenetin, exhibited significant, concentration-dependent inhibitory effects on lipid accumulation in 3T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2'-dihydroxy-4',5',6'-trimethoxychalcone, which caused 75% and 90% inhibition of cellular lipid accumulation at 30 and 50 µmol/L, respectively. Both kaempferide and 4,2'-dihydroxy-4',5',6'-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant. C. odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accumulation in 3T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 065693577

Download citation: RISBibTeXText

PMID: 30352773

DOI: 10.1016/j.joim.2018.10.002

Related references

Ecology and management of Chromolaena odorata: proceedings of the second international workshop on biological control of Chromolaena odorata. Biotrop Special Publication ( 44): 167 pp., 1991

Flavonoids of Chromolaena odorata. Phytochemistry 17(10): 1807-1808, 1978

Antimycobacterial activity and cytotoxicity of flavonoids from the flowers of Chromolaena odorata. Archives of Pharmacal Research 27(5): 507-511, 2004

Exudate flavonoids in a tropical weed, Chromolaena odorata (L.) R. M. King et H. Robinson. Biochemical Systematics and Ecology 23(7/8): 873-874, 1995

Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage. Burns 27(4): 319-327, 2001

Effect of a 60-day oral gavage of a crude alkaloid extract from Chromolaena odorata leaves on hormonal and spermatogenic indices of male rats. Journal of Andrology 33(6): 1199-1207, 2014

A New Flavanone from the Leaves of Chromolaena odorata. Natural Product Communications 10(9): 1555-1559, 2015

Effect of essential oils from leaves of Ageratum conyzoides, Lantana camara and Chromolaena odorata on the mortality of Sitophilus zeamais (Coleoptera, Curculionidae). Journal of Stored Products Research 37(2): 103-109, 2001

Wound healing activity of Chromolaena odorata leaves. Journal of Tropical Medicinal Plants 8(1): 9-13, 2007

A New Flavanone as a Potent Antioxidant Isolated from Chromolaena odorata L. Leaves. Evidence-Based Complementary and Alternative Medicine 2019: 1-12, 2019

The after effect of filtered sunlight through green leaves on the seed germination of Chromolaena odorata (L.) R.M. King and H. Robinson compared with that of sunlight and darkness. Proceedings of the Third Indonesian Weed Science Conference, Bandung 1975: 12-17, 1975

Phytochemical constituents and antioxidant properties of extracts from the leaves of Chromolaena odorata. 2007

Flavonoid glycosides from Chromolaena odorata leaves and their in vitro cytotoxic activity. Chemical and Pharmaceutical Bulletin 59(1): 129-131, 2011

Ethanol extract of lotus (Nelumbo nucifera) root exhibits an anti-adipogenic effect in human pre-adipocytes and anti-obesity and anti-oxidant effects in rats fed a high-fat diet. Nutrition Research 34(3): 258-267, 2014

Platelet-activating factor (PAF) receptor binding antagonist activity of the methanol extracts and isolated flavonoids from Chromolaena odorata (L.) King and Robinson. Biological and Pharmaceutical Bulletin 30(6): 1150-1152, 2007