+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles

A ratiometric detection of heparin with high sensitivity based on aggregation-enhanced emission of gold nanoclusters triggered by silicon nanoparticles

Talanta 193: 37-43

Heparin (Hep) is a widely applied anticoagulant and the quantification of heparin concentration is pivotal for clinical use. In this work, silicon nanoparticles (SiNPs) modified by the amino groups and glutathione-capped gold nanoclusters (GSH-AuNCs) are able to self-assemble into spherical particle structures via the electrostatic interaction, resulting in the aggregation-enhanced emission (AEE) of GSH-AuNCs. However, Hep, a highly sulfated glycosaminoglycan with much more negative charges, can bind with the SiNPs and inhibit the aggregation. As a result, it causes the AEE quenching of GSH-AuNCs at 570 nm but the SiNPs keep their own blue fluorescence at 450 nm. Thus, the SiNPs can act as an internal reference and the GSH-AuNCs are used as a signal probe in this process. The ratiometric fluorescent signal (I570/I450) change of the nanohybrid probe is positively correlated with Hep concentrations in the range from 6.44 ng/mL to 96.6 ng/mL with the detection limit of 3.29 ng/mL. As expected, this strategy shows good sensitivity and selectivity, and it is also successfully applied to detect Hep in Hep sodium injection and human serum samples with good recoveries.

(PDF emailed within 0-6 h: $19.90)

Accession: 065699003

Download citation: RISBibTeXText

PMID: 30368295

DOI: 10.1016/j.talanta.2018.09.098

Related references

Aggregation-induced emission enhancement of gold nanoclusters triggered by silicon nanoparticles for ratiometric detection of protamine and trypsin. Analytica Chimica Acta 1046: 170-178, 2018

Ratiometric fluorescence detection of trace water in organic solvents based on aggregation-induced emission enhanced Cu nanoclusters. Analyst 143(13): 3068-3074, 2018

A novel ratiometric fluorescence nanoprobe based on aggregation-induced emission of silver nanoclusters for the label-free detection of biothiols. Talanta 188: 623-629, 2018

Highly sensitive and selective dual-emission ratiometric fluorescence detection of dopamine based on carbon dots-gold nanoclusters hybrid. Sensors and Actuators B: Chemical 265: 371-377, 2018

Aggregation-Enhanced Emission of Gold Nanoclusters Induced by Serum Albumin and Its Application to Protein Detection and Fabrication of Molecular Logic Gates. Acs Omega 3(10): 12763-12769, 2018

Aggregation-induced emission of luminol: a novel strategy for fluorescence ratiometric detection of ALP and As(v) with high sensitivity and selectivity. Chemical Communications 54(54): 7487-7490, 2018

Sensitive fluorescence detection of heparin based on self-assembly of mesoporous silica nanoparticle-gold nanoclusters with emission enhancement characteristics. Analyst 143(22): 5388-5394, 2018

Gold nanoclusters-based dual-emission ratiometric fluorescence probe for monitoring protein kinase. Sensors and Actuators B: Chemical 226: 144-150, 2016

Reusable Silicon-Based Surface-Enhanced Raman Scattering Ratiometric Aptasensor with High Sensitivity, Specificity, and Reproducibility. Analytical Chemistry 89(19): 10279-10285, 2017

Naked eye detection of infertility based on sperm protamine-induced aggregation of heparin gold nanoparticles. Analytical and Bioanalytical Chemistry 410(13): 3053-3058, 2018

Colorimetric detection of L-histidine based on the target-triggered self-cleavage of swing-structured DNA duplex-induced aggregation of gold nanoparticles. Mikrochimica Acta 185(10): 452, 2018

Near-infrared dual-emission quantum dots-gold nanoclusters nanohybrid via co-template synthesis for ratiometric fluorescent detection and bioimaging of ascorbic acid in vitro and in vivo. Analytical Chemistry 87(19): 9998-10005, 2016

Aggregation-induced emission from gold nanoclusters for use as a luminescence-enhanced nanosensor to detect trace amounts of silver ions. Journal of Colloid and Interface Science 467: 90-96, 2016

A pyrene-based fluorescent sensor for ratiometric detection of heparin and its complex with heparin for reversed ratiometric detection of protamine in aqueous solution. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy 170: 198-205, 2018

Redox-Triggered Bonding-Induced Emission of Thiol-Functionalized Gold Nanoclusters for Luminescence Turn-On Detection of Molecular Oxygen. Acs Sensors 2(11): 1692-1699, 2017