EurekaMag
+ Translate
+ Most Popular
Allopurinol and Loss of Consciousness in a 78-old Year Man Suffering from Gout
The parasites of British birds and mammals. 3XIV-3XVH
Studies on the in vitro synthesis of phi x 174 super helical covalently closed circular duplex dna and circular single stranded dna
The Shahada movement: a peasant movement in Maharashtra (India) - its development and its perspectives
The cranial nerves of Wallago attu
Anatomy of Mystus seenghala IV Nervous system
Haematology of Persian fallow deer (Dama mesopotamica)
Major weeds of rabi and kharif crops and their control
Structure of Morija-Banol iron-ore deposits, Rajasthan, and its control over mineralization
Donkioporia expansa - A lesser known wood destroyer in buildings
The new physiology of vision 3Xin. The testing of color-vision
Economic importance of Pteridophytes
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Ketoconazole shampoo 1% vs. climbazole shampoo in the treatment of seborrhoeic scalp dermatitis
Economic efficiency of biwall drip irrigation in sugarcane production - a case study in Ankalkhop village in Sangli District of Maharashtra
48,4X-49,5X mosaic: asynchronies among the late-replicating X chromosomes
Compatibility of potassium phosphonate (Akomin-40) with different species of Trichoderma and Gliocladium virens
Enterobacter amnigenus. An unusual human pathogen
The 48 4X 49 4Xy 49 4X iso chromosome yq mosaicism in a 3 year old boy from a twin pregnancy
Placentrex treatment in corneal defects in animals
The biology of human starvation: some new insights
Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA
Replenishable but depletable resources: water
New insights into bestiality and zoophilia
Thoracic outlet syndrome in Ehlers-Danlos patients as displayed by magnetic resonance imaging, angiography and venography

Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to Biocides


Behavior of Foodborne Pathogens Listeria monocytogenes and Staphylococcus aureus in Mixed-Species Biofilms Exposed to Biocides



Applied and Environmental Microbiology 84(24)



ISSN/ISBN: 0099-2240

PMID: 30291117

DOI: 10.1128/aem.02038-18

In nature and man-made environments, microorganisms reside in mixed-species biofilms, in which the growth and metabolism of an organism are different from these behaviors in single-species biofilms. Pathogenic microorganisms may be protected against adverse treatments in mixed-species biofilms, leading to health risk for humans. Here, we developed two mixed five-species biofilms that included one or the other of the foodborne pathogens Listeria monocytogenes and Staphylococcus aureus The five species, including the pathogen, were isolated from a single food-processing environmental sample, thus mimicking the environmental community. In mature mixed five-species biofilms on stainless steel, the two pathogens remained at a constant level of ∼105 CFU/cm2 The mixed five-species biofilms as well as the pathogens in monospecies biofilms were exposed to biocides to determine any pathogen-protective effect of the mixed biofilm. Both pathogens and their associate microbial communities were reduced by peracetic acid treatments. S. aureus decreased by 4.6 log cycles in monospecies biofilms, but the pathogen was protected in the five-species biofilm and decreased by only 1.1 log cycles. Sessile cells of L. monocytogenes were affected to the same extent when in a monobiofilm or as a member of the mixed-species biofilm, decreasing by 3 log cycles when exposed to 0.0375% peracetic acid. When the pathogen was exchanged in each associated microbial community, S. aureus was eradicated, while there was no significant effect of the biocide on L. monocytogenes or the mixed community. This indicates that particular members or associations in the community offered the protective effect. Further studies are needed to clarify the mechanisms of biocide protection and to identify the species playing the protective role in microbial communities of biofilms.IMPORTANCE This study demonstrates that foodborne pathogens can be established in mixed-species biofilms and that this can protect them from biocide action. The protection is not due to specific characteristics of the pathogen, here S. aureus and L. monocytogenes, but likely caused by specific members or associations in the mixed-species biofilm. Biocide treatment and resistance are a challenge for many industries, and biocide efficacy should be tested on microorganisms growing in biofilms, preferably mixed systems, mimicking the application environment.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 065710493

Download citation: RISBibTeXText

Related references

Growth Inhibition of Foodborne Pathogens in Camel Milk: Staphylococcus aureus, Listeria monocytogenes, Salmonella spp. and E. coli O157:H7. Czech Journal of Food Sciences 35(4): 311-320, 2017

Bactericidal activities of health-promoting, food-derived powders against the foodborne pathogens Escherichia coli, Listeria monocytogenes, Salmonella enterica, and Staphylococcus aureus. Journal of Food Science 78(2): M270-M275, 2013

Interactions in dual species biofilms between Listeria monocytogenes EGD-e and several strains of Staphylococcus aureus. International Journal of Food Microbiology 126(1-2): 76-82, 2008

Survival of foodborne pathogens (Escherichia coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus, Listeria monocytogenes, and Vibrio parahaemolyticus) in raw ready-to-eat crab marinated in soy sauce. International Journal of Food Microbiology 238: 50-55, 2016

Effect of peracetic acid on biofilms formed by Staphylococcus aureus and Listeria monocytogenes isolated from dairy plants. Journal of Dairy Science 99(3): 2384-2390, 2016

Double-Pipette Method for Selection of Motile Foodborne Pathogens ( Listeria Monocytogenes or Salmonella Typhimurium ) from Mixed Culture Systems. Journal of Rapid Methods and Automation in Microbiology 7(1): 1-6, 1999

Removal of Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157:H7 biofilms on stainless steel using scallop shell powder. Food Control 25(1): 1-9, 2012

Capillary electrophoresis separation of protein composition of γ-irradiated food pathogens Listeria monocytogenes and Staphylococcus aureus. Plos one 7(3): E32488, 2012

Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes. Pathogens 9(5), 2020

Performance of stress resistant variants of Listeria monocytogenes in mixed species biofilms with Lactobacillus plantarum. International Journal of Food Microbiology 213: 24-30, 2015

Behavior of Listeria monocytogenes and Staphylococcus aureus in yogurt fermented with a bacteriocin-producing thermophilic starter. Journal of Food Protection 65(5): 799-805, 2002

Spatial competition with Lactococcus lactis in mixed-species continuous-flow biofilms inhibits Listeria monocytogenes growth. Biofouling 27(9): 1065-1072, 2011

Current Knowledge on Listeria monocytogenes Biofilms in Food-Related Environments: Incidence, Resistance to Biocides, Ecology and Biocontrol. Foods 7(6), 2018

Mixed species biofilms of Listeria monocytogenes and Lactobacillus plantarum show enhanced resistance to benzalkonium chloride and peracetic acid. International Journal of Food Microbiology 144(3): 421-431, 2011

Behavior of Salmonella spp., Listeria monocytogenes, and Staphylococcus aureus in Chouriço de Vinho, a dry fermented sausage made from wine-marinated meat. Journal of Food Protection 76(4): 588-594, 2013