+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Comparative analysis of the pathogenicity between multidrug-resistant Acinetobacter baumannii clinical isolates: isolation of highly pathogenic multidrug-resistant A. baumannii and experimental therapeutics with fourth-generation cephalosporin cefozopran



Comparative analysis of the pathogenicity between multidrug-resistant Acinetobacter baumannii clinical isolates: isolation of highly pathogenic multidrug-resistant A. baumannii and experimental therapeutics with fourth-generation cephalosporin cefozopran



Infection and Drug Resistance 11: 1715-1722



The pathogenicity of fatal-outbreak Acinetobacter baumannii isolates has not been fully investigated. This study aimed to compare the pathogenicity between A. baumannii clinical isolates, including multidrug-resistant A. baumannii (MDRA). Antibiotic susceptibility was determined by the broth microdilution method, and drug-resistant genes were characterized by PCR and sequencing. The pathogenicity of A. baumannii and antibiotic responses were evaluated using the Galleria mellonella infection model. Clinical isolates from an A. baumannii outbreak at our hospital were categorized using the pulse-field gel electrophoresis. Of the 16 isolated A. baumannii clones, 12 clones were resistant to carbapenems (meropenem and imipenem), of which 10 clones were also resistant to amikacin and ciprofloxacin (MDRAs). MDRAs had OXA-51-like β-lactamase gene harboring an insertion sequence in the promoter region and armA gene encoding 16S rRNA methyltransferase. Carbapenem- and/or amikacin-resistant A. baumannii were more pathogenic than carbapenem- and/or amikacin-sensitive A. baumannii in G. mellonella. MDRA isolate TK1033 was more virulent than other A. baumannii isolates. However, TK1033 was sensitive to the fourth-generation cephalosporin cefozopran in addition to minocycline, tigecycline, and polymyxins (colistin and polymyxins B) in vitro and in vivo in the MDRA-G. mellonella infection model. Differences in pathogenicity among carbapenem-resistant A. baumannii clones are consistent with heterogeneous clinical outcomes. Strain TK1033, isolated frequently during the outbreak, was the most virulent, whereas preoutbreak isolate TK1032 was less virulent than other A. baumannii isolates. Infection by high-virulence isolates may be more prevalent during outbreaks. These strains may prove valuable for investigating MDRA virulence and novel therapeutics.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 065733508

Download citation: RISBibTeXText

PMID: 30349328

DOI: 10.2147/idr.s166154


Related references

Characterization, sequencing and comparative genomic analysis of vB_AbaM-IME-AB2, a novel lytic bacteriophage that infects multidrug-resistant Acinetobacter baumannii clinical isolates. Bmc Microbiology 14: 181, 2014

Comparative assessment of antimicrobial susceptibility testing for tigecycline and colistin against Acinetobacter baumannii clinical isolates, including multidrug-resistant isolates. International Journal of Antimicrobial Agents 44(5): 396-401, 2014

Minocycline activity against clinical isolates of multidrug-resistant Acinetobacter baumannii. Clinical Microbiology and Infection 21(11): E79-E80, 2015

In vitro activity of tigecycline against multidrug-resistant Acinetobacter baumannii clinical isolates. Le Infezioni in Medicina 17(4): 236-239, 2009

Draft Genome Sequences of Clinical Isolates of Multidrug-Resistant Acinetobacter baumannii. Genome Announcements 5(5):, 2017

Next-Generation Sequencing and Comparative Analysis of Sequential Outbreaks Caused by Multidrug-Resistant Acinetobacter baumannii at a Large Academic Burn Center. Antimicrobial Agents and ChemoTherapy 60(3): 1249-1257, 2015

Highly Synergistic Effects of Melittin with Conventional Antibiotics Against Multidrug-Resistant Isolates of Acinetobacter baumannii and Pseudomonas aeruginosa. Microbial Drug Resistance 25(2): 193-202, 2019

Antibiotic resistance determinants of multidrug-resistant Acinetobacter baumannii clinical isolates in Algeria. Diagnostic Microbiology and Infectious Disease 76(4): 529-531, 2013

Study on the resistant genes to carbapenems and epidemiological characterization of multidrug-resistant Acinetobacter baumannii isolates. Microbial Drug Resistance 19(2): 117-123, 2013

Molecular characterization of carbapenem-non-susceptible Acinetobacter spp. in Japan: predominance of multidrug-resistant Acinetobacter baumannii clonal complex 92 and IMP-type metallo-β-lactamase-producing non-baumannii Acinetobacter species. Journal of Infection and ChemoTherapy 18(4): 522-528, 2012

Extracellular proteome of a highly invasive multidrug-resistant clinical strain of Acinetobacter baumannii. Journal of Proteome Research 11(12): 5678-5694, 2012

Evaluation of in Vitro Tigecycline Activity Against Multidrug-Resistant Acinetobacter Baumannii Clinical Isolates from Poland. Acta Poloniae Pharmaceutica 74(3): 793-800, 2017

Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii. Journal of Bacteriology 190(24): 8053-8064, 2008

Comparative activities of polycationic peptides and clinically used antimicrobial agents against multidrug-resistant nosocomial isolates of Acinetobacter baumannii. Journal of Antimicrobial Chemotherapy 46(5): 807-810, 2000

Comparative in vitro activities of tigecycline and 11 other antimicrobial agents against 215 epidemiologically defined multidrug-resistant Acinetobacter baumannii isolates. Journal of Antimicrobial ChemoTherapy 58(5): 1099-1100, 2006