+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Complete genome sequence of the marine fish pathogen Vibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential for in vivo infection



Complete genome sequence of the marine fish pathogen Vibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential for in vivo infection



Microbiological Research 216: 97-107



Vibrio anguillarum is a notorious bacterial pathogen that causes vibriosis in various marine farmed fish species. The highly pathogenic V. anguillarum strain MVM425 has been isolated from moribund turbot in the China Yellow Sea, but the genetic basis for its pathogenesis is undefined. Complete genome sequencing uncovered that the bacterium encodes 3985 protein-coding sequences (CDSs) on two chromosomes and 62 CDSs on the virulence plasmid pEIB1. To investigate the genes that are essential for its in vitro and in vivo growth, we applied transposon insertion sequencing technology (Tn-seq) to screen a highly saturated transposon insertion mutant library grown in Luria-Bertani broth with 2% NaCl (LB20) and in its natural host turbot. Tn-seq identified 473 and ∼173 putative genes as essential for its growth in rich medium and the in vivo infection process, respectively. Additionally, our analysis revealed that the genes are associated to various fitness levels in the liver, spleen and kidney, suggesting different genetic requirements for colonization in these organs. Furthermore, we validated our Tn-seq data using gene knockout mutants and in vivo infection experiments. Comprehensive functional genomics analysis highlighted the conditionally essential genes for important pathways involved in energy metabolism, nitrogen metabolism, nucleotide synthesis, amino acid synthesis, cofactors, siderophore synthesis, secretion (T1SS, T2SS, and T6SS), quorum sensing, and flagellum biosynthesis. This study provides a wealth of information about V. anguillarum genes related to infection in fish and will facilitate the understanding of its pathogenesis as well as the development of diagnostics and vaccines against the pathogen.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 065736450

Download citation: RISBibTeXText

PMID: 30269861

DOI: 10.1016/j.micres.2018.08.011


Related references

Complete genome sequence of the marine fish pathogen Vibrio anguillarum harboring the pJM1 virulence plasmid and genomic comparison with other virulent strains of V. anguillarum and V. ordalii. Infection and Immunity 79(7): 2889-2900, 2011

Complete sequence of virulence plasmid pJM1 from the marine fish pathogen Vibrio anguillarum strain 775. Journal of Bacteriology 185(19): 5822-5830, 2003

Genome-wide transposon mutagenesis of Proteus mirabilis: Essential genes, fitness factors for catheter-associated urinary tract infection, and the impact of polymicrobial infection on fitness requirements. Plos Pathogens 13(6): E1006434, 2017

Complete sequence of virulence plasmid pEIB1 from the marine fish pathogen Vibrio anguillarum strain MVM425 and location of its replication region. Journal of Applied Microbiology 97(5): 1021-1028, 2004

Genome-wide sequence transposon insertion sites and analyze the essential genes of Brucella melitensis. Microbial Pathogenesis 112: 97-102, 2017

Complete genome sequence analysis of the fish pathogen Flavobacterium columnare provides insights into antibiotic resistance and pathogenicity related genes. Microbial Pathogenesis 111: 203-211, 2017

Complete genome sequence of Vibrio parahaemolyticus strain FORC_008, a foodborne pathogen from a flounder fish in South Korea. Pathogens and Disease 74(5):, 2016

Complete Genome Sequence of Vibrio anguillarum Nontailed Bacteriophage NO16. Microbiology Resource Announcements 8(15):, 2019

Complete Genome Sequence of Vibrio anguillarum M3, a Serotype O1 Strain Isolated from Japanese Flounder in China. Genome Announcements 1(5):, 2013

Complete genome sequence of Vibrio anguillarum strain NB10, a virulent isolate from the Gulf of Bothnia. Standards in Genomic Sciences 10: 60, 2015

Complete genome sequence and comparative analysis of the fish pathogen Lactococcus garvieae. Plos one 6(8): E23184, 2011

Genome-wide investigation of the genes involved in nicotine metabolism in Pseudomonas putida J5 by Tn5 transposon mutagenesis. Applied Microbiology and Biotechnology 99(15): 6503-6514, 2015

Genome-wide mining of potential virulence-associated genes in Riemerella anatipestifer using random transposon mutagenesis. Veterinary Microbiology 189: 52-58, 2016

Molecular analysis of r plasmids isolated from strains of the marine fish pathogen vibrio anguillarum. Abstracts of the Annual Meeting of the American Society for Microbiology 83: H84, 1983

The complete genome sequence and comparative genome analysis of the multi-drug resistant food-borne pathogen Bacillus cereus. Genomics 2019:, 2019