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Evaluation of the Vitek-MS™ system in the identification of Candida isolates from bloodstream infections


Evaluation of the Vitek-MS™ system in the identification of Candida isolates from bloodstream infections



Revista Iberoamericana de Micologia 35(3): 130-133



ISSN/ISBN: 1130-1406

PMID: 30343836

DOI: 10.1016/j.riam.2018.02.001

Matrix-assisted laser desorption-time of flight mass spectrometry (MALDI-TOF-MS) represents a revolution in the identification of microorganisms of clinical interest. Many studies have confirmed the accuracy and fastness of this tool with routine strains. To identify clinical isolates of Candida from patients diagnosed with candidemia. Vitek-MS™ system was used with a collection of 298 blood isolates of the genus Candida represented by 9 different species. Sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA cluster was used as the reference method. The results of Vitek-MS™ were concordant with those obtained with the reference method for 279 (93.62%) isolates (Kappa coefficient (κ)=0.91). Vitek-MS™ misidentified 10 (3.36%) isolates and did not identify 9 (3.02%) isolates. This study determines the potential of Vitek-MS™ in yeast identification, being a reliable and fast alternative in the clinical laboratory, with an acceptable sensitivity of 82% (IC 95%: 70-90.6%), in comparison with a 100% (IC 95%: 92.9-100%) sensitivity of the conventional methods.

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Accession: 065780075

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