+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Glucosamine improves survival in a mouse model of sepsis and attenuates sepsis-induced lung injury and inflammation

Glucosamine improves survival in a mouse model of sepsis and attenuates sepsis-induced lung injury and inflammation

Journal of Biological Chemistry 2018

The aim of the current study was to investigate the effects of glucosamine (GlcN) on septic lethality and sepsis-induced inflammation using animal models of mice and zebrafish. GlcN pretreatment improved survival in the cecal ligation and puncture (CLP)-induced sepsis mouse model and attenuated LPS-induced septic lung injury and systemic inflammation. GlcN suppressed LPS-induced M1-specific but not M2-specific gene expression. Furthermore, increased expressions of inflammatory genes in visceral tissue of LPS-injected zebrafish were suppressed by GlcN. GlcN suppressed LPS-induced activation of MAPKs and NF-κB in lung tissue. LPS triggered a reduction in O-GlcNAc levels in nucleocytoplasmic proteins of lung, liver and spleen after 1 day, which returned to normal levels on days 3. GlcN inhibited LPS-induced O-GlcNAc downregulation in mouse lung and visceral tissue of zebrafish. Furthermore, O-GlcNAcase (OGA) level was increased by LPS, which were suppressed by GlcN in mouse and zebrafish. OGA inhibitors suppressed LPS-induced expression of inflammatory genes in RAW264.7 cells and the visceral tissue of zebrafish. Stable knockdown of OGA via shRNA led to increased iNOS expression in response to LPS with or without GlcN in RAW264.7 cells. Overall, our results demonstrate a protective effect of GlcN on sepsis potentially through modulation of O-GlcNAcylation of nucleocytoplasmic proteins.

(PDF emailed within 0-6 h: $19.90)

Accession: 065797083

Download citation: RISBibTeXText

PMID: 30455348

DOI: 10.1074/jbc.RA118.004638

Related references

Ulinastatin attenuates liver injury and inflammation in a cecal ligation and puncture induced sepsis mouse model. Journal of Cellular Biochemistry 120(1): 417-424, 2018

Niacin attenuates lung inflammation and improves survival during sepsis by downregulating the nuclear factor-κB pathway. Critical Care Medicine 39(2): 328-334, 2011

Inhibition of necroptosis attenuates lung injury and improves survival in neonatal sepsis. Surgery 2018, 2018

Alpha-chemokine receptor blockade reduces high mobility group box 1 protein-induced lung inflammation and injury and improves survival in sepsis. American Journal of Physiology. Lung Cellular and Molecular Physiology 289(4): L583-L590, 2005

A novel anti-histone H1 monoclonal antibody, SSV monoclonal antibody, improves lung injury and survival in a mouse model of lipopolysaccharide-induced sepsis-like syndrome. Biomed Research International 2015: 491649, 2015

Glutamine attenuates lung injury and improves survival after sepsis: role of enhanced heat shock protein expression. Critical Care Medicine 33(6): 1206-1213, 2005

Luteolin attenuates acute lung injury in experimental mouse model of sepsis. Cytokine 110: 333-343, 2018

Tanshinone IIA attenuates sepsis-induced immunosuppression and improves survival rate in a mice peritonitis model. Biomedicine and PharmacoTherapy 112: 108609, 2019

Tityus serrulatus scorpion venom improves survival and lung inflammation in lethal sepsis induced by CLP in mice. Toxicon 89: 1-8, 2015

Cystine improves survival rates in a LPS-induced sepsis mouse model. Clinical Nutrition 34(6): 1159-1165, 2016

Antagonism of the Neurokinin-1 Receptor Improves Survival in a Mouse Model of Sepsis by Decreasing Inflammation and Increasing Early Cardiovascular Function. Critical Care Medicine 45(2): E213-E221, 2016

Human kallistatin administration reduces organ injury and improves survival in a mouse model of polymicrobial sepsis. Immunology 142(2): 216-226, 2014

PP279-SUN: Cystine Improves Survival Rate in LPS-Induced Sepsis Mouse Model. Clinical Nutrition 33: S123-S124, 2014

Effect of pentoxifylline on lung inflammation and gas exchange in a sepsis-induced acute lung injury model. Brazilian Journal of Medical and Biological Research 39(11): 1455-1463, 2006

Alternate day calorie restriction improves systemic inflammation in a mouse model of sepsis induced by cecal ligation and puncture. Journal of Surgical Research 174(1): 136-141, 2012