EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Inhibition of silkworm vacuolar-type ATPase activity by its inhibitor Bafilomycin A1 induces caspase-dependent apoptosis in an embryonic cell line of silkworm


Inhibition of silkworm vacuolar-type ATPase activity by its inhibitor Bafilomycin A1 induces caspase-dependent apoptosis in an embryonic cell line of silkworm



Archives of Insect Biochemistry and Physiology 99(3): E21507



ISSN/ISBN: 0739-4462

PMID: 30246413

DOI: 10.1002/arch.21507

Vacuolar-type ATPase (V-ATPase) is a type of hydrogen ion transporter located in the vesicular membrane-like system, which mediates active transport and intracellular acidification in various compartments. In mammals, V-ATPase has been reported to play a key role in cell proliferation and apoptosis. The studies of V-ATPase in silkworm mainly focus on the acidification regulation of midgut and silk gland and immune resistance. However, there are few reports about the function of silkworm V-ATPase on cell proliferation, autophagy, and apoptosis. Thus, the function of V-ATPase in a cell line of Bombyx mori (BmE) was investigated by treating the cell line with bafilomycin A1, a specific inhibitor of V-ATPase. Cell counting kit 8 (CCK8) and flow cytometry analysis showed that bafilomycin A1 treatment decreased the cell proliferation activity, affected the cell cycle progression and induced cell apoptosis. LysoTracker Red staining showed that the target of bafilomycin A1 is lysosome. The expression of all autophagy-related genes ( BmATG5, BmATG6, and BmATG8) decreased, indicating that cell autophagy was inhibited. The analysis of the apoptosis pathway demonstrated that inhibiting the activity of V-ATPase of BmE cells could promote mitochondria to release cytochrome C, inhibit the expression of BmIAP, and activate the caspase cascade to induce apoptosis. All these findings systematically illustrate the effects of V-ATPase on the proliferation, autophagy, and apoptosis in BmE cells, and provide new ideas and a theoretical basis for further study on the function of V-ATPase in BmE.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 065818714

Download citation: RISBibTeXText

Related references

Effects of bafilomycin A1, a vacuolar type H+ ATPase inhibitor, on the thermosensitivity of a human pancreatic cancer cell line. International Journal of Hyperthermia: the Official Journal of European Society for Hyperthermic Oncology North American Hyperthermia Group 22(4): 275-285, 2006

Bafilomycin A-1, a potent selective inhibitor of vacuolar H+-ATPase, induces apoptosis in PC12 cells. Cell Structure and Function 19(6): 541, 1994

Inhibition of Cell Growth by Bafilomycin A1, a Selective Inhibitor of Vacuolar H+-Atpase. In Vitro Cellular and Developmental Biology. Animal 29(11): 862-866, 1993

Inhibition of cell growth by bafilomycin A1, a selective inhibitor of vacuolar H(+)-ATPase. In Vitro Cellular and Developmental Biology. Animal 29(11): 862-866, 1993

Blockade of vacuolar proton ATPase by bafilomycin A1 induces neuronal apoptosis in murine cortical cell cultures. Society for Neuroscience Abstracts 25(1-2): 1827, 1999

Vacuolar H+-ATPase inhibitor induces apoptosis via lysosomal dysfunction in the human gastric cancer cell line MKN-1. Journal of Biochemistry 134(3): 359-364, 2003

Caspase-3 activation during the process of apoptosis induced by a vacuolar type H(+)-ATPase inhibitor. Biology of the Cell 91(7): 507-513, 1999

Bafilomycin A1, a specific vacuolar-type H+-ATPase inhibitor blocks the action of tetanus toxin in spinal cord neurons in cell culture. Molecular Biology of the Cell 4(Suppl ): 325A, 1993

Cell-type and amyloid precursor protein-type specific inhibition of Ab release by bafilomycin A1, a selective inhibitor of vacuolar ATPases. The Journal of Biological Chemistry 270: 2, 1995

Cell-type and amyloid precursor protein-type specific inhibition of a beta release by bafilomycin A1, a selective inhibitor of vacuolar ATPases. Journal of Biological Chemistry 270(6): 2419-2422, 1995

Increase in Bcl-2 level promoted by CD40 ligation correlates with inhibition of B cell apoptosis induced by vacuolar type H(+)-ATPase inhibitor. Experimental Cell Research 238(1): 82-89, 1998

Induction of phagocytic activity of M1 cells by an inhibitor of vacuolar H+-ATPase, bafilomycin A1. Febs Letters 337(3): 221-225, 1994

IMP dehydrogenase inhibitor mycophenolate mofetil induces caspase-dependent apoptosis and cell cycle inhibition in multiple myeloma cells. Molecular Cancer Therapeutics 5(2): 457-466, 2006

Analyses of the early processes of autophagy using bafilomycin a 1 a specific inhibitor of vacuolar type proton atpase. Cell Structure and Function 16(6): 615, 1991

Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, blocks lysosomal cholesterol trafficking in macrophages. Journal of Biological Chemistry 268(36): 27345-27348, 1993