+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

MicroRNA-301b promotes cell proliferation and apoptosis resistance in triple-negative breast cancer by targeting CYLD



MicroRNA-301b promotes cell proliferation and apoptosis resistance in triple-negative breast cancer by targeting CYLD



Bmb Reports 51(11): 602-607



Aberrant expression of microRNAs (miRNAs) plays important roles in carcinogenesis and tumor progression. However, the expression and biological role of miR-301b in triple-negative breast cancer (TNBC) remains unclear. Here we aimed to evaluate the roles and mechanisms of miR-301b in TNBC cells. miR-301b expression was assessed in TNBC specimens and cell lines by quantitative Real-Time PCR (qRT-PCR). TNBC cells were transfected with miR-301b mimics, inhibitors or Cylindromatosis (CYLD) small interfering RNA (siRNA) using Lipofectamine 2000. The functional roles of miR-301b were determined by cell proliferation, colony formation, and apoptosis assays. Western blots and qRT-PCR were used to measure the expression of mRNAs and proteins in the cells. We found that miR-301b was upregulated in TNBC specimens and cell lines. Overexpression of miR-301b promoted cell proliferation in TNBC cells, while inhibited the apoptosis induced by 5-FU. CYLD was downregulated by miR-301b at both mRNA and protein levels in TNBC cells. Dual-luciferase report assay confirmed that miR-301b downregulated CYLD by direct interaction with the 3'-untranslated region(3'-UTR) of CYLD mRNA. NF-κB activation was mechanistically associated with miR-301b-mediated downregulation of CYLD. However, inhibition of miR-301b reversed all the effects of miR-301b. In conclusion, miR-301b plays an oncogenic role in TNBC possibly by downregulating CYLD and subsequently activating NF-κB p65, and this may provide a novel therapeutic approach for TNBC. [BMB Reports 2018; 51(11): 602-607].

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 065845085

Download citation: RISBibTeXText

PMID: 30269739


Related references

MicroRNA-182 promotes proliferation and metastasis by targeting FOXF2 in triple-negative breast cancer. Oncology Letters 14(4): 4805-4811, 2017

MicroRNA‑124 suppresses cell proliferation and invasion of triple negative breast cancer cells by targeting STAT3. Molecular Medicine Reports 19(5): 3667-3675, 2019

MicroRNA-32 promotes cell proliferation, migration and suppresses apoptosis in breast cancer cells by targeting FBXW7. Cancer Cell International 17: 14, 2017

MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells. Journal of Experimental and Clinical Cancer Research 31: 58, 2012

MiR-4458 regulates cell proliferation and apoptosis through targeting SOCS1 in triple-negative breast cancer. Journal of Cellular Biochemistry 120(8): 12943-12948, 2019

MicroRNA‑27a promotes tumorigenesis via targeting AKT in triple negative breast cancer. Molecular Medicine Reports 17(1): 562-570, 2018

Jagged2 promotes cancer stem cell properties of triple negative breast cancer cells and paclitaxel resistance via regulating microRNA-200. European Review for Medical and Pharmacological Sciences 22(18): 6008-6014, 2018

Exosomal MicroRNA MiR-1246 Promotes Cell Proliferation, Invasion and Drug Resistance by Targeting CCNG2 in Breast Cancer. Cellular Physiology and Biochemistry 44(5): 1741-1748, 2017

MiR-25-3p promotes the proliferation of triple negative breast cancer by targeting BTG2. Molecular Cancer 17(1): 4, 2018

MicroRNA-455-3p promotes invasion and migration in triple negative breast cancer by targeting tumor suppressor EI24. Oncotarget 8(12): 19455-19466, 2017

MiR-135b promotes proliferation and metastasis by targeting APC in triple-negative breast cancer. Journal of Cellular Physiology 234(7): 10819-10826, 2019

Long non-coding RNA MALAT1 promotes proliferation and invasion via targeting miR-129-5p in triple-negative breast cancer. Biomedicine and PharmacoTherapy 95: 922-928, 2017

FOXP2 Promotes Tumor Proliferation and Metastasis by Targeting GRP78 in Triple-negative Breast Cancer. Current Cancer Drug Targets 18(4): 382-389, 2018

MiRNA-21 promotes proliferation and invasion of triple-negative breast cancer cells through targeting PTEN. American Journal of Translational Research 9(3): 953-961, 2017

MicroRNA-223 Increases the Sensitivity of Triple-Negative Breast Cancer Stem Cells to TRAIL-Induced Apoptosis by Targeting HAX-1. Plos one 11(9): E0162754, 2016