+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The contribution of fast growing, psychrotrophic microorganisms on biodiversity of refrigerated raw cow's milk with high bacterial counts and their food spoilage potential

The contribution of fast growing, psychrotrophic microorganisms on biodiversity of refrigerated raw cow's milk with high bacterial counts and their food spoilage potential

Food Microbiology 79: 11-19

Storage of raw milk in the bulk tank creates an environment which selects for psychrotrophic bacteria. Results from earlier studies suggested that the microbiota of bulk tank milk with high bacterial counts is dominated by single, cold-adapted species with high growth rates at low temperatures. We checked this assumption in more detail and analyzed the microbial diversity of 48 samples from bulk tank raw cow's milk with bacterial counts >100,000 cfu/mL from different geographic regions by culture-dependent and -independent methods. Contrary to presumptions from earlier studies, only the minority (24%) of samples was dominated by a single bacterial species and diversity was not correlated with bacterial counts. The dominating species in this group of samples were identified as psychrotrophic Acinetobacter and Pseudomonas species, related to poor hygiene and spoilage, or mesophilic, mastitis-related Streptococcus species and Escherichia coli. This shows that storage of raw milk under refrigeration does not always lead to a selection of cold-adapted bacteria. Approximately half of the raw milk isolates showed either lipolytic or proteolytic activity at 10 °C or 4 °C. Consistent or increased enzymatic activity at cold temperatures was detected for Acinetobacter spp. and Pseudomonas spp., but also for genera with minor abundance, e.g. Carnobacterium and Arthrobacter.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 066070898

Download citation: RISBibTeXText

PMID: 30621865

DOI: 10.1016/j.fm.2018.10.019

Related references

Biodiversity of refrigerated raw milk microbiota and their enzymatic spoilage potential. International Journal of Food Microbiology 211: 57-65, 2015

Note. Differences in level of contamination by psychrotrophic microorganisms and psychrotrophic enterobacteria in refrigerated pasteurized milk according to the type of packaging and period of marketing. Revista de Agroquimica y Tecnologia de Alimentos 30(3): 397-402, 1990

The main spoilage-related psychrotrophic bacteria in refrigerated raw milk. Journal of Dairy Science 101(1): 75-83, 2018

Psychrotrophic microorganisms in refrigerated pasteurized milk. Brief Communications of the XXIII International Dairy Congress, Montreal, October 8-12, 1990, Vol I: 119, 1990

Rapid estimation of psychrotrophic and proteolytic bacterial counts from total bacterial counts in cooled raw milk. International Journal of Food Science & Technology 23(4): 379-384, 1988

Influence of psychrotrophic microorganisms on the quality of refrigerated milk. A review. Higiene Alimentar 12(55): 21-27, 1998

Preventing psychrotrophic bacterial spoilage in raw milk. United States Patent (US 4 912 047): 5 pp., 1990

Determination of total counts of psychrotrophic bacteria in pool milk samples and their relation to total counts of microorganisms. Czech Journal of Food Sciences 17(6): 216-222, 1999

Verification of the proteolytic and lipolytic activities of the microbial flora isolated from raw, refrigerated, type B milk. II. Psychrotrophic microorganisms. Revista Latinoamericana de Microbiologia 41(2): 85-89, 2000

Inhibition of psychrotrophic bacterial growth in refrigerated milk by addition of carbon dioxide. Journal of Dairy Science 71(1): 52-60, 1988

Hydrolytic potential of a psychrotrophic Pseudomonas isolated from refrigerated raw milk. Brazilian Journal of Microbiology 42(4): 1479-1484, 2011

Psychrotrophic and total bacterial counts in raw milk. Veterinarstvi 48(9): 373-374, 1998

Inhibitory activity of adipic acid on food spoilage microorganisms studies on growth inhibition of food spoilage microorganisms for low salt foods part vi. Journal of the Japanese Society for Food Science & Technology (Nippon Shokuhin Kogyo Gakkaishi) 34(2): 88-93, 1987

Quorum Sensing and Spoilage Potential of Psychrotrophic Enterobacteriaceae Isolated from Milk. Biomed Research International 2018: 2723157, 2018

Spoilage potential of psychrotrophic bacteria isolated from raw milk and the thermo-stability of their enzymes. Journal of Zhejiang University. Science. B 19(8): 630-642, 2018