+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014



Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011-2014



Food Microbiology 79: 116-122



The presence of Listeria monocytogenes was evaluated in a small-scale meat processing facility in Montenegro during 2011-2014. L. monocytogenes isolates from traditional meat products and environmental swabs were subjected to a) molecular characterization b) serotyping by both multiplex PCR and next generation sequencing (NGS) c) potential antimicrobial resistance (AMR) was assessed by extraction of specific genes from NGS data and d) screening for the presence of some disinfectant resistance markers. Overall, traditional meat products were contaminated, most likely from incoming raw materials, with 4 major specific STs of L. monocytogenes (ST515, ST8, ST21, ST121) representing 4 clonal complexes (CC1, CC8, CC21, CC121) identified during the four-year period. These strains belonged to serogroup IIa which predominated, followed by IVb (ST515, CC1). The strains from environmental swabs belonged, exclusively, to ST21 and were isolated from cutting board and floor swabs in 2011. Furthermore, we found Tn6188, a novel transposon conferring tolerance to BC, to be specific to sequence type ST121. In addition, antimicrobial resistance genes mprF and fosX were present in clonal complexes CC21 and CC121, while complexes CC8 and CC1 exclusively harbored the mprF antimicrobial resistance gene.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066070899

Download citation: RISBibTeXText

PMID: 30621866

DOI: 10.1016/j.fm.2018.12.005


Related references

A first molecular characterization of Listeria monocytogenes isolates circulating in humans from 2009 to 2014 in the Italian Veneto region. New Microbiologica 41(3): 232-234, 2018

Characterization of Listeria monocytogenes isolates from 50 small-scale Austrian cheese factories. Journal of Food Protection 69(6): 1297-1303, 2006

Molecular ecology of Listeria monocytogenes and other Listeria species in small and very small ready-to-eat meat processing plants. Journal of Food Protection 74(1): 63-77, 2011

Prevalence of Listeria spp. and Molecular Characterization of Listeria monocytogenes Isolates from Broilers at the Abattoir. Foodborne Pathogens and Disease 12(7): 606-611, 2015

Characterization of Listeria monocytogenes isolates from the meat, poultry and seafood industries by automated ribotyping. International journal of food microbiology 72(1-2): 137-146, 2002

Evaluation of 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes for the Detection of Listeria monocytogenes in Selected Foods and Environmental Surfaces: Collaborative Study, First Action 2014.07. Journal of Aoac International 98(4): 980-992, 2015

Molecular characterization of Listeria monocytogenes isolates from ready-to-eat foods. Abstracts of the General Meeting of the American Society for Microbiology 103: P-123, 2003

Molecular epidemiology of Listeria monocytogenes isolates collected from the environment, raw meat and raw products in two poultry- and pork-processing plants. Journal of applied microbiology 91(5): 888-899, 2001

Serological and molecular ecology of Listeria monocytogenes isolates collected from 13 French pork meat salting-curing plants and their products. International Journal of Food Microbiology 112(2): 153-161, 2006

Distribution of Listeria monocytogenes molecular subtypes among human and food isolates from New York State shows persistence of human disease--associated Listeria monocytogenes strains in retail environments. Journal of Food Protection 67(7): 1417-1428, 2004

Serogrouping and pulsed-field gel electrophoresis analysis of Listeria monocytogenes isolates from cases of human infection in Hungary 2004–2012 molecular typing of Listeria monocytogenes in Hungary. Acta Microbiologica et Immunologica Hungarica 61(1): 71-78, 2014

Antimicrobial Resistance, Virulence Profile, and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China, 2013-2014. Biomedical and Environmental Sciences 29(6): 448-452, 2017

MLVA subtyping of Listeria monocytogenes isolates from meat products and meat processing plants. Food Research International 106: 225-232, 2018

MLVA subtyping of Listeria monocytogenes isolates from meat products and meat processing plants. Food Research International 106: 225-232, 2018

Prevalence of Listeria monocytogenes in raw meats marketed in Bangkok and characterization of the isolates by phenotypic and molecular methods. Journal of Health Population and Nutrition 29(1): 26-38, 2011