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A molecular technique for selection of self-compatible varieties of Japanese pear (Pyrus pyrifolia Nakai) - Screening of self-compatible variety by PCR-RFLP

A molecular technique for selection of self-compatible varieties of Japanese pear (Pyrus pyrifolia Nakai) - Screening of self-compatible variety by PCR-RFLP

Euphytica 138(1): 73-80

ISSN/ISBN: 0014-2336

DOI: 10.1023/b:euph.0000047075.01110.86

The pear cultivar 'Osa-Nijisseiki' (S2S4sm; sm = stylar-part mutant) has been used as a parent to breed self-compatible cultivars that produce excellent fruits. However, determination of the self-compatibility of 'Osa-Nijisseiki' offspring requires a lot of time, 6 years or more, by conventional cross breeding. We have designed a rapid reliable method for the identification of self-compatible varieties of 'Osa-Nijisseiki' offspring based on the polymerase chain reaction (PCR) and restriction fragment length polymorphism ( RFLP) with S-allele specific restriction endonucleases. By using this method, 8 self-compatible varieties were selected among 16 selections resulting from a cross between the self-compatible cultivar 'Osa-Nijisseiki' (S2S4sm) and the self-incompatible cultivars 'Niitaka' (S3S9), 'Whasan' (S3S5), 'Chuwhangbae' (S4S6). The S-genotypes of 16 'Osa-Nijisseiki' offsprings were also determined.

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Accession: 066187537

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