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Direct rhizogenesis and establishment of fast growing normal root organ culture of Withania somnifera Dunal

Direct rhizogenesis and establishment of fast growing normal root organ culture of Withania somnifera Dunal

Plant Cell Tissue and Organ Culture 84(2): 223-225

ISSN/ISBN: 0167-6857

Direct rooting from leaf explants of Withania somnifera was achieved on half strength Murashige and Skoogs medium supplemented with 15 g l(-1) sucrose, and different concentrations of growth regulators. Basal medium supplemented with 2.85 mu M indoleacetic acid and 9.85 mu M indolebutyric acid achieved maximum number of roots with 100% response. The roots were cultured on MS liquid medium for the establishment of root-organ culture with the same plant growth regulators and incubated on an orbital shaker at 80 rpm at 25 +/- 2 degrees C. A root biomass of 6.15 +/- 0.17 g was obtained after 5 weeks. When 1 g roots were inoculated to 2.51 bubble column reactor, 47 g roots were obtained after 6 weeks. The concentration of alkaloids was increased as compared to field grown roots. The maximum concentration of withanolides (10 mg g(-1) dry weight) was obtained in the bioreactor.

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Accession: 066195323

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