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Multiplex detection of Potato virus S, Potato virus X, and Potato Virus Y by non-radioactive nucleic acid spot hybridization in potato tissue culture plantlets


Multiplex detection of Potato virus S, Potato virus X, and Potato Virus Y by non-radioactive nucleic acid spot hybridization in potato tissue culture plantlets



American Journal of Potato Research 83(6): 495-501



ISSN/ISBN: 1099-209X

DOI: 10.1007/bf02883510

Potato plantlets initiated into tissue culture must be tested for numerous viruses prior to propagation for seed potato production. Ideally, one plantlet is tested for all pathogens of concern and, if found pathogen-free, this plantlet is propagated for production of seed potatoes. Commercially available ELISA kits are generally used for the pathogen tests, but the commercial kits have some limitations. For example, the protocols differ for different viruses, so multiple extractions must be completed, increasing the time and expense of testing. This is a significant problem with tissue culture plantlets, for which there is limited material available to test and an ever-increasing number of pathogens that must be tested for, including viruses in the potyvirus, carlavirus, potexvirus, luteovirus, pomovirus, tobravirus, tospovirus, alfamovirus, and tymovirus groups. We have optimized a non-radioactive nucleic acid hybridization (NASH) assay for the simultaneous detection of carlavirus Potato virus S (PVS), potexvirus Potato virus X (PVX) and potyvirus Potato virus Y (PVY) in potato tissue culture plantlets. This assay requires a single extraction from a small portion of a tissue culture plantlet for the detection of viruses from three different families.

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Accession: 066200253

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