+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn

+ Translate
+ Recently Requested

Escherichia coli O157 : H7-secreted cytotoxins are toxic to enterocytes and increase Escherichia coli O157 : H7 colonization of jejunum and descending colon in cattle



Escherichia coli O157 : H7-secreted cytotoxins are toxic to enterocytes and increase Escherichia coli O157 : H7 colonization of jejunum and descending colon in cattle



Canadian Journal of Animal Science 88(1): 41-50



Enterohemorrhagic Escherichia coli (EHEC) O157:H7-secreted cytotoxins are toxic to target cells and enhance colonization of intestinal tissues in disease-susceptible animals. It is unclear what role, if any, EHEC O157:H7-secreted cytotoxins play in the colonization of intestinal tissues of healthy reservoir animals. We previously reported that EHEC O157:H7 colonization sites were associated with focal hemorrhages in the jejunum and descending colon of persistent shedding cattle, suggesting a potential role for cytotoxins in EHEC O157:H7 colonization. We have used a traditional EHEC O157:H7 IVOC adherence assay and a novel lawn assay to examine the role of EHEC O157:H7-secreted cytotoxins in EHEC O157:H7 strain colonization of the jejunum and descending colon of non-persistent and persistent shedding cattle. Four EHEC O157:H7 strains that were previously reported to differentially colonize cattle produced cytotoxins that were differentially active against epithelial cells from the jejunum and descending colon. There was a relationship between EHEC O157:H7-secreted cytotoxin activity and strain adherence for epithelial cells from the jejunum and descending colon of cattle. There was also a greater susceptibility of epithelial cells from the jejunum and descending colon to EHEC O157:H7-secreted cytotoxins of persistent shedding cattle compared with non-persistent shedding cattle. Addition of the most active secreted cytotoxins from EHEC O157:H7 R318N to the IVOC adherence assays significantly increased the adherence of the most (R318N) and least (H4420N) virulent EHEC O157: H7 strain to intestinal tissues. The current study supports a role for EHEC O157:H7-secreted cytotoxins in enhancing EHEC O157:H7 colonization of intestinal tissues of cattle.

(PDF emailed within 0-6 h: $19.90)

Accession: 066209713

Download citation: RISBibTeXText

DOI: 10.4141/CJAS07043



Related references

Escherichia coli O157H7-secreted cytotoxins are toxic to enterocytes and increase Escherichia coli O157H7 colonization of jejunum and descending colon in cattle. Canadian Journal Of Animal Science: 1, 41-50, 2008

A rapid, sensitive method for testing the activity of Escherichia coli O157 : H7 secreted cytotoxins against epithelial cells from the jejunum and descending colon of cattle. Canadian Journal of Animal Science 88(1): 51-55, 2008

Heterogeneity in enterohemorrhagic Escherichia coli O157:H7 fecal shedding in cattle is related to Escherichia coli O157:H7 colonization of the small and large intestine. Canadian Journal of Microbiology 54(12): 984-995, 2009

Use of Nonpathogenic Escherichia coli Surrogates as Predictors of the Survival of Nontyphoidal Salmonella, non-O157 Shiga Toxin-Producing Escherichia coli, and Escherichia coli O157 Populations after High Hydrostatic Pressure Processing. Journal of Food Protection 81(7): 1068-1072, 2018

Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey. Journal of Veterinary Science 11(4): 321-326, 2011

Competition for proline between indigenous Escherichia coli and E. coli O157:H7 in gnotobiotic mice associated with infant intestinal microbiota and its contribution to the colonization resistance against E. coli O157:H7. Antonie van Leeuwenhoek 94(2): 165-171, 2008

Fecal shedding of non-O157 serogroups of Shiga toxin-producing Escherichia coli in feedlot cattle vaccinated with an Escherichia coli O157:H7 SRP vaccine or fed a Lactobacillus-based direct-fed microbial. Journal of Food Protection 77(5): 732-737, 2014

Evaluation of lactic acid as an initial and secondary subprimal intervention for Escherichia coli O157:H7, non-O157 Shiga toxin-producing E. coli, and a nonpathogenic E. coli surrogate for E. coli O157:H7. Journal of Food Protection 75(9): 1701-1708, 2012

Gene markers of generic Escherichia coli associated with colonization and persistence of Escherichia coli O157 in cattle. Preventive Veterinary Medicine 117(1): 140-148, 2015

British Escherichia coli O157 in Cattle Study (BECS): to determine the prevalence of E. coli O157 in herds with cattle destined for the food chain. Epidemiology and Infection 145(15): 3168-3179, 2017

Effect of a vaccine product containing type III secreted proteins on the probability of Escherichia coli O157 : H7 fecal shedding and mucosal colonization in feedlot cattle. Journal of Food Protection 70(11): 2568-2577, 2007

Evaluation of hha and hha sepB mutant strains of Escherichia coli O157:H7 as bacterins for reducing E. coli O157:H7 shedding in cattle. Vaccine 29(31): 5078-5086, 2011

Thermotolerance of Escherichia coli O157:H7 ATCC 43,894, Escherichia coli B, and an rpoS-deficient mutant of Escherichia coli O157:H7 ATCC 43,895 following exposure to 1.5% acetic acid. Journal of Food Protection 61(9): 1184-1186, 1998

Escherichia coli O157:H7 and non-O157 Shiga toxin-producing E. coli in healthy cattle, sheep and swine herds in Northern Spain. Zoonoses and Public Health 55(2): 73-81, 2008

Efficacy of Escherichia coli O157:H7 siderophore receptor/porin proteins-based vaccine in feedlot cattle naturally shedding E. coli O157. Foodborne Pathogens and Disease 6(7): 893-899, 2009