+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Introgression of useful linked genes for resistance to stem rust, leaf rust and powdery mildew and their molecular validation in wheat (Triticum aestivum L.)



Introgression of useful linked genes for resistance to stem rust, leaf rust and powdery mildew and their molecular validation in wheat (Triticum aestivum L.)



Indian Journal of Genetics and Plant Breeding 69(1): 17-27



Two independent DNA segments with two linked genes each namely, Lr19/Sr25 and Sr36/Pm6 were introgressed by three cycles of backcrossing into fifteen bread wheat (Triticum, aestivum L.) cultivars, susceptible to leaf rust:, (U), stem rust (Sr) and powdery mildew (Pm). Evaluation of the BC3-F-5 along with their recurrent parents and controls carrying specific Lr and Sr genes under natural and artificial conditions indicated that conventionally improved newly constituted lines confer resistance to leaf rust, stem rust and powdery mildew in adult stage against the pathotypes prevailing in the Nilgirls. Some of these lines also displayed resistance to leaf rust pathotype 77-8 (253R 31) in adult stage. However, the improved lines, viz., C306*(3)/Cook*(6)/C80-1, HD2687*(3)/Cook*(6)/C80-1, MACS2496*(3)/Cook*(6)/C80-1, PBW226*(3)/Cook*(6)/C80-1, UP2338*(3)/Cook*(6)/C80-1 and UP262*(3)/Cook*(6)/C80-1 showed moderate susceptibility to 77-8. The leaf rust pathotype 77-8 was found avirulent on Lr26. The resistance in UP262 can be ascribed to Lr23 and some unknown gene(s) possessed by it. Since Lr19 is effective to all the pathotypes of leaf rust In India except 77-8, the differential response exhibited by BC3-F-5 lines against a mixture of prevailing races at Wellington and against a new pathotype 77-8 at New Delhi indicated unambiguously the presence of Lr19 in the introgressed lines. High degree of resistance to stem rust and powdery mildew In all the BC3-F-5 lines evidenced that these carry the genes Sr36 and Pm6. The conventional methodology proved successful in phenotype-based selection of resistance gene combinations In the absence of molecular markers. To ensure the correct identity of target genes, the BC3-F-5 lines (2 to 9) were tested with molecular markers SCS265, SCS253 and Gb linked to leaf rust resistance gene Lr19 and SSR stm773 for stem rust resistance gene Sr36. The presence of genetically associated genes was verified by the amplification of translocated DNA segment in most of the backcross lines. With the detection and testing with a new Lr19 virulence, host-pathogen interaction test response against leaf rust and the molecular test response did not correspond in HW2004*(3)/Cook*(6)/C80-1 because of presence of an additional segment of Lt24/Sr24. The availability of a combination of major genetically diverse resistance genes in adapted wheat cultivars would facilitate the strategic deployment to achieve enhanced durable resistance. Also these lines could be used as donor and supplement the molecular mapping and tagging of genes.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 066220574

Download citation: RISBibTeXText


Related references

Stable linked genes for rust and powdery mildew resistance in wheat (Triticum aestivum). Indian Journal of Agricultural Sciences 76(12): 721-725, 2006

Identification of powdery mildew and leaf rust resistance genes in common wheat (Triticum aestivum L.). Wheat varieties from the Caucasus, Central and Inner Asia. Genetic Resources and Crop Evolution 51(4): 355-370, 2004

Identification of powdery mildew and leaf rust resistance genes in common wheat (Triticum aestivum L. em. Thell.) cultivars grown in Bulgaria and Russia. Plant Genetic Resources Newsletter ( 122): 32-35, 2000

Transfer of leaf rust and powdery mildew resistance genes from Triticum durum to Triticum aestivum. Genetika a Slechteni 33(1): 13-20, 1997

Resistance of Tangmai 4 wheat to powdery mildew, stem rust, leaf rust, and stripe rust and its chromosome composition. Canadian Journal of Plant Science 84(4): 1015-1023, 2004

Introgression of leaf rust and stripe rust resistance from Sharon goatgrass (Aegilops sharonensis Eig) into bread wheat (Triticum aestivum L.). Genome 57(6): 309-316, 2014

Validation and incorporation of leaf rust resistance genes Lr9, Lr19, Lr24 and Lr26 through molecular markers in wheat (Triticum aestivum L.). 2007

Introgression lines obtained from the cross between Triticum aestivum and Triticum turgidum (durum wheat) as a source of leaf and stripe (yellow) rust resistance genes. Turkish Journal of Biology 40(3): 547-553, 2016

Transfer to hexaploid wheat of linked genes for adult plant leaf rust and seedling stem rust resistance from an amphiploid of aegilops speltoides x triticum monococcum. Genome 33(4): 530-537, 1990

Transfer to hexaploid wheat of linked genes for adult-plant leaf rust and seedling stem rust resistance from an amphiploid of Aegilops speltoides Triticum monococcum. Genome 33(4): 530-537, 1990

Genetics of resistance to wheat leaf rust, stem rust, and powdery mildew in Aegilops sharonensis. Phytopathology 98(3): 353-358, 2008

An evaluation of 391 spring wheat triticum aestivum introductions for resistance to stem rust and leaf rust. Canadian Journal of Plant Pathology 8(2): 132-139, 1986

Inheritance and interaction of genes governing reaction to stem rust, leaf rust and powdery mildew in a spring Wheat cross. J. Amer. Soc. Agron, 36: 12, 991-992, 1944

A multiple resistance locus on chromosome arm 3BS in wheat confers resistance to stem rust (Sr2), leaf rust (Lr27) and powdery mildew. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 123(4): 615-623, 2011

Resistance in Aegilops species against leaf rust, stem rust, Septoria tritici blotch, eyespot and powdery mildew of wheat. Zeitschrift fuer Pflanzenkrankheiten und Pflanzenschutz 105(6): 624-631, 1998