Cybrid/hybrid plants regenerated from somatic fusions between male sterile Satsuma mandarin and seedy tangelos

Cai, X.; Fu, J.; Chen, C.; Guo, W.

Scientia Horticulturae 122(2): 323-327


ISSN/ISBN: 0304-4238
DOI: 10.1016/j.scienta.2009.05.017
Accession: 066222681

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Somatic hybridization provides an alternative for transferring mitochondria-encoded cytoplasmic male sterility (CMS). Herein, symmetric protoplast electrofusion was conducted between embryogenic callus protoplasts of Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a CMS cultivar, and mesophyll-derived protoplasts of seedy 'Page' tangelo [C. reticulata Blanco x (C. reticulata Blanco x C. paradisi Macf.)] or 'Nova' tangelo [C. reticulata Blanco x (C. reticulata Blanco x C. paradisi Macf.)], to transfer CMS trait. Flow cytometry analysis showed that 14 plants recovered from G1 + 'Page' tangelo that displayed typical morphological character of 'Page' were diploid, and 6 plants regenerated from G1 + 'Nova' tangelo were tetraploid. Genetic compositions of regenerated plants from the two fusions were determined by SSR, CAPS and chloroplast-SSR analysis. Cybrid nature of diploids from G1 + 'Page' tangelo with nuclear DNA from 'Page', mitochondrial DNA (mtDNA) from the G1 and chloroplast DNA (cpDNA) derived from either parent was confirmed. Tetraploid plants from G1 + 'Nova' tangelo were identified as somatic hybrids with random cpDNA inheritance. The regenerated cybrid and hybrid plants hold great potential for Citrus seedless breeding at diploid or triploid levels.