+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Molecular characterization through IGS sequencing of formae speciales of Fusarium oxysporum pathogenic on lamb's lettuce



Molecular characterization through IGS sequencing of formae speciales of Fusarium oxysporum pathogenic on lamb's lettuce



Phytopathologia Mediterranea 49(3): 309-320



Twenty-nine strains of Fusarium oxysporum isolated from wilted lamb's lettuce plants (Valerianella olitoria) along with eight ATCC reference strains were examined for differences in the nucleotide sequences of the ribosomal DNA (rDNA) intergenic spacer (IGS) region, which is about 2.5 kb long in these strains. A phylogenetic (neighbour-joining) analysis was performed on the strains and identified four phylogenetic groups, I, II, III, and IV. Most F. oxysporum isolates recovered from wilted lamb's lettuce plants cultivated in northern Italy clustered in group I and were very similar to F. oxysporum f. sp. raphani. All the isolates, including the 8 control strains, were tested for pathogenicity on lamb's lettuce cv. Trophy cultivated in the glasshouse. Most isolates from northern Italy were pathogenic on lamb's lettuce, displaying varying degrees of virulence, and their IGS sequence were similar to the forma specialis raphani. However, four isolates were not pathogenic on lamb's lettuce. Among the control strains, six showed moderate virulence; these belonged to the formae speciales raphani and conglutinans; and two, belonging to the forma specialis matthioli (ATCC16602 and ATCC16603) were not pathogenic on lamb's lettuce. In conclusion, analysis of the IGS sequences indicated that the isolates studied had different origins, and that phylogeny and pathogenicity were related; non-pathogenic isolates differed genetically from isolates that were poorly, moderately or highly virulent. To the best of our knowledge, this is the first report on the differences between formae speciales of F. oxysporum on lamb's lettuce plants as determined by IGS sequence analysis.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 066242519

Download citation: RISBibTeXText


Related references

Molecular characterization through IGS sequencing of formae speciales of Fusarium oxysporum pathogenic on lamb's lettuce and rocket. Phytopathology 101(6): S65-S66, 2011

Infection and pathological histology of pigeon pea plants inoculated with pathogenic and non-pathogenic formae speciales of Fusarium oxysporum. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 82(8/9): 485-492, 1975

Hyphal ramification in pigeonpea of two non-pathogenic formae speciales of Fusarium oxysporum. Journal of Mycology and Plant Pathology 30(2): 210-215, 2000

Vegetative compatibility grouping of formae speciales of Fusarium oxysporum pathogenic to the Liliaceae. Nauchno-Tekhnicheskii Byulleten' Vsesoyuznogo Selektsionno-Geneticheskogo Instituta 59(1): 3-9, 1993

Tomatinase induction in formae speciales of Fusarium oxysporum non-pathogenic of tomato plants. Physiological & Molecular Plant Pathology 50(1): 37-52, 1997

Interactions of the tomato d lycopersicon esculentum d with 2 formae speciales of fusarium oxysporum fusarium oxysporum f sp lycopersici fusarium oxysporum f sp pisi. Annals of Applied Biology 62(3): 413-427, 1968

Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest Management Science 64(8): 781-788, 2008

Inhibition of wilt symptoms by Fusarium oxysporum f. sp. udum in pigeon pea (Cajanus cajan) induced by other formae speciales of Fusarium oxysporum. Phytopathologia Mediterranea 16(1): 1-4, 1977

Fusarium oxysporum f sp cucurbitacearum embracing all formae speciales of fusarium oxysporum attacking cucurbitaceous crops. Netherlands Journal of Plant Pathology 94(1): 17-31, 1988

Characterization of the formae speciales of Fusarium oxysporum causing wilts of cucurbits by DNA fingerprinting with nuclear repetitive DNA sequences. Applied and Environmental Microbiology 60(8): 2684-2691, 1994

Diseases of flowering and ornamental plants, new or little known in Italy. IV. The presence in Italy of two formae speciales of Fusarium oxysporum pathogenic on chrysanthemums. Informatore Fitopatologico 25(11): 9-12, 1975

Inhibition mechanisms of indole derivatives against Fusarium oxysporum f.sp. spinaciae and different sensitivity of formae speciales of F. oxysporum to the compounds. Proceedings of the Kansai Plant Protection Society ( 42): 3-8, 2000

Distribution of the FoToml gene encoding tomatinase in formae speciales of Fusarium oxysporum and identification of a novel tomatinase from F. oxysporum f. sp. radicis-lycopersici, the causal agent of Fusarium crown and root rot of tomato. Journal of General Plant Pathology 70(4): 195-201, 2004

Quantitative comparison of nuclear DNA content among formae speciales of Fusarium oxysporum and Fusarium solani. Research Bulletin of the Faculty of Agriculture, Gifu University (51): 29-33, 1986

Endopolygalacturonase PG1 in Different Formae Speciales of Fusarium oxysporum. Applied and Environmental Microbiology 64(5): 1967-1971, 1998