+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers



Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers



Electronic Journal of Biotechnology 14(3): 14



Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes (Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four differential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using molecular markers. Eleven different Lr genes were postulated in the material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26, Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedling resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41, showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a + Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number of virulent reactions against local pathotypes. Occurrence of adult plant resistance genes Lr34, Lr35 and Lr37 in local germplasm was evaluated using specific molecular markers confirming presence of Lr34 and Lr37. Our data suggest that combinations including seedling resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with adult plant resistance genes like Lr34, SV2, Lr46 will probably provide durable and effective resistance to leaf rust in the region.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066247512

Download citation: RISBibTeXText

DOI: 10.2225/vol14-issue3-fulltext-14


Related references

Postulation of seedling leaf rust resistance genes in selected Ethiopian and German bread wheat cultivars. Crop Science 48(2): 507-516, 2008

Identification of leaf rust resistance genes in selected egyptian wheat cultivars by molecular markers. Thescientificworldjournal 2014: 574285, 2014

Identification of leaf rust resistance genes in wheat (Triticum aestivum L.) cultivars using molecular markers. Genetika 42(5): 675-683, 2006

Gene postulation of resistance genes to leaf rust (Puccinia triticina Ericks.) on seedling and adult plant of bread wheat (Triticum aestivum L.) elite genotypes. Revista Mexicana de Fitopatologia 21(3): 239-247, 2003

Postulation and Mapping of Seedling Stripe Rust Resistance Genes in ETHIOPIAN BREAD WHEAT CULTIVARS. Journal of Plant Pathology 94(2): 403-409, 2012

Postulation of genes (Lr) for resistance to leaf rust in Brazilian wheat cultivars. Fitopatologia Brasileira 27(5): 508-516, 2002

Postulation of Leaf Rust Resistance Genes in Seven Chinese Spring Wheat Cultivars. Journal of Integrative Agriculture 12(9): 1580-1588, 2013

Postulation of leaf rust resistance genes in 47 new wheat cultivars (lines) at seedling stage. Scientia Agricultura Sinica 40(9): 1925-1935, 2007

Postulation of leaf rust resistance genes in Iranian wheat cultivars and breeding lines. Canadian Journal of Plant Pathology 33(4): 550-558, 2011

Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars. Journal of Integrative Agriculture 14(10): 1992-2001, 2015

Identification of molecular markers associated with partial resistance to powdery mildew, leaf rust and stripe rust in bread wheat line Saar. Options Mediterraneennes: A, Seminaires Mediterraneens, 2008

Postulation of leaf (brown) rust resistance genes in 70 wheat cultivars grown in the United Kingdom. Euphytica 120(2): 205-218, 2001

Identification of stem rust resistance genes in wheat cultivars in China using molecular markers. Peerj 6: E4882, 2018

Identification of leaf rust resistance genes in common wheat by molecular markers. Acta Phytopathologica et Entomologica Hungarica 35(1/4): 31-36, 2000

Molecular markers linked to the leaf rust resistance gene Lr24 in different wheat cultivars. Genome 39(5): 830-835, 1996