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Genetic Diversity in Faba Bean (Vicia faba L.) Using Inter-Simple Sequence Repeat (ISSR) Markers and Protein Analysis



Genetic Diversity in Faba Bean (Vicia faba L.) Using Inter-Simple Sequence Repeat (ISSR) Markers and Protein Analysis



Life Science Journal-Acta Zhengzhou University Overseas Edition 9(2): 497-503



The present study aims to assess the genetic diversity among 10 varied faba bean cultivars, collected from two wide-ranging geographical locations of Egypt. Variability based on the DNA level was inspected through nine ISSR-PCR screening, which showed obvious differences among the various Vicia faba (V. faba) cvs. A total of 576 ISSR loci were detected and 398 (69.10%) of them were polymorphic, which represent a relatively high polymorphism level. Cluster analysis via ISSR markers separated three green large-seeded cvs. (V. faba var. major) from dry small-seeded cvs. (V. faba var. minor). The small-seeded cvs. were further classified into two sub-clusters according to two geographic locations. The first sub-cluster included dry small-seeded cvs. grown under clay soil conditions (Abies location). However, the second sub-cluster integrated the similar dry small-seeded cvs. but were grown under calcareous soil conditions (Fuka location). SDS-PAGE analysis of various faba bean leaf proteins reflected some variations among studied V. faba populations. The results clarified that ISSR markers and protein analysis were helpful to recognize genetic variation among faba bean cultivars. [H. S. Abdel-Razzak, A. M. Alfrmawy, H. M. Ibrahim and Amr. A. El-Hanafy. . Life Sci J 2012;9(2):497-503]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 74

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