Survey of single strand conformation polymorphism of kappa-casein gene in Alpine and Saanen goats in Iran

Nejhad, R.K.; Yousefi, S.; Azari, M.A.

Animal Science Papers and Reports 31(2): 173-177


ISSN/ISBN: 0860-4037
Accession: 066273033

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To study the genetic polymorphism of K-casein (CSN3) gene as candidate gene for marker-assisted selection in different domestic livestock species, blood samples were randomly withdrawn from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene was amplified by polymerase chain reaction (PCR). PCR products were analysed using single strand conformation polymorphism (SSCP) method. Results indicated that there were two (K-1 and K-2) conformational patterns with frequencies 0.91 and 0.09 for Alpine and 0.76 and 0.24 for Saanen goats, respectively. A Chi-square test confirmed Hardy-Weinberg equilibrium for the gene in both breeds.