+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The effect of LPS on LH release and gene expression of LH-beta, GnRH-R and TLR4 in the anterior pituitary of follicular phase ewes - an in vitro study



The effect of LPS on LH release and gene expression of LH-beta, GnRH-R and TLR4 in the anterior pituitary of follicular phase ewes - an in vitro study



Journal of Animal and Feed Sciences 22(2): 97-105



The study was designed to determine the in vitro effect of bacterial endotoxin - lipopolysaccharide (LPS) - on luteinizing hormone (LH) secretion from anterior pituitary (AP) explants collected from saline-treated 'healthy' and LPS-treated ewes in the follicular phase. In the AP explants, the expression of LH-beta, gonadotropin-releasing hormone receptor (GnRH-R) and Toll-like receptor 4 (TLR4) genes was also assayed. It was found that explants incubated alone with LPS, as well as together with LPS-binding protein (LBP), abolished the stimulatory effect of GnRH on LH release. LPS added without LBP did not suppress LH-beta gene expression in 'healthy' APs, however. Moreover, LBP intensified the inhibitory effect of LPS on gene expression in 'healthy' APs. These results show that LPS is a potent negative modulator of LH secretion and suggest that its direct action on the pituitary gland could be one of the mechanisms via which an immune/inflammatory challenge inhibits the reproductive process.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 066273793

Download citation: RISBibTeXText


Related references

The central effect of beta-endorphin and naloxone on the expression of GnRH Gene and GnRH receptor (GnRH-R) gene in the hypothalamus, and on GnRH-R gene in the anterior pituitary gland in follicular phase ewes. Experimental and Clinical Endocrinology and Diabetes 116(1): 40-46, 2008

Effect of stress on the expression of GnRH and GnRH receptor (GnRH-R) genes in the preoptic area-hypothalamus and GnRH-R gene in the stalk/median eminence and anterior pituitary gland in ewes during follicular phase of the estrous cycle. Acta Neurobiologiae Experimentalis 67(1): 1-12, 2007

Effects of GABA(A) receptor modulation on the expression of GnRH gene and GnRH receptor (GnRH-R) gene in the hypothalamus and GnRH-R gene in the anterior pituitary gland of follicular-phase ewes. Animal Reproduction Science 111(2-4): 235-248, 2009

Expression of the GnRH and GnRH receptor (GnRH-R) genes in the hypothalamus and of the GnRH-R gene in the anterior pituitary gland of anestrous and luteal phase ewes. Animal Reproduction Science 108(3-4): 345-355, 2008

Effects of corticotropin-releasing hormone and its antagonist on the gene expression of gonadotrophin-releasing hormone (GnRH) and GnRH receptor in the hypothalamus and anterior pituitary gland of follicular phase ewes. Reproduction Fertility and Development 23(6): 780-787, 2011

Expression of the GnRH and GnRH receptor genes in the hypothalamus and of the GnRH-R gene in the anterior pituitary gland of anestrous and luteal phase ewes. Animal Reproduction Science8: 3 4, 345-355, 2008

The Central Effect of β-Endorphin and Naloxone on The Biosynthesis of GnRH and GnRH Receptor (GnRHR) in The Hypothalamic-Pituitary Unit of Follicular-Phase Ewes. Reproduction in Domestic Animals 51(4): 555-561, 2016

Implication of dopaminergic systems on GnRH and GnRHR genes expression in the hypothalamus and GnRH-R gene expression in the anterior pituitary gland of anestrous ewes. Experimental and Clinical Endocrinology and Diabetes 116(6): 357-362, 2008

Effect of endotoxin on the expression of GnRH and GnRHR genes in the hypothalamus and anterior pituitary gland of anestrous ewes. Animal Reproduction Science 120(1-4): 105-111, 2010

Effect of neuropeptide Y on GnRH-induced LH release from bovine anterior pituitary cell cultures derived from heifers in a follicular, luteal or ovariectomized state. Animal Reproduction Science 78(1/2): 25-31, 2003

Relationship between gonadotrophin subunit gene expression gonadotrophin releasing hormone receptor content and pituitary and plasma gonadotrophin concentrations during the rebound release of fsh after treatment of ewes with bovine follicular fluid during the luteal phase of the cycle. Journal of Molecular Endocrinology 8(2): 109-118, 1992

Changes in the GnRH mRNA and GnRH receptor (GnRH-R) mRNA levels in the hypothalamic-anterior pituitary unit of anestrous ewes after infusion of GnRH into the third cerebral ventricle. Reproductive Biology 8(2): 149-161, 2008

The effect of stress on the expression of GnRH and GnRH receptor genes in the discrete regions of the hypothalamus and pituitary of anestrous ewes. Reproductive Biology 7(1): 55-71, 2007

The role of GABAA receptors in the neural systems of the medial preoptic area in the control of GnRH release in ewes during follicular phase. Animal Reproduction Science 77(1/2): 71-83, 2003

Activation of central GABAA-but not of GABAB-receptors rapidly reduces pituitary LH release and GnRH gene expression in the preoptic/anterior hypothalamic area of ovariectomized rats. Neuroendocrinology 61(6): 655-662, 1995