+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Optimization of Callus Induction and Plant Regeneration in Sugarcane (Saccharum spp.) for a Study of Sucrose Accumulation in Relation to Soluble Acid Invertase Expression

Optimization of Callus Induction and Plant Regeneration in Sugarcane (Saccharum spp.) for a Study of Sucrose Accumulation in Relation to Soluble Acid Invertase Expression

Chiang Mai Journal of Science 42(4): 797-805

The response of three sugarcane genotypes with differential sucrose accumulation to MS medium + 2, 4-dichlorophenoxyacetic acid (2, 4-D) and/or kinetin (kn) was investigated. On MS + 2, 4-D (2 mg/l) + kn (0.5 mg/l), Louisiana Purple (LAP) had the highest callus induction (95.80 %), followed by H65-7052 (82.68 %) and Molokai (MOL) (62.58 %). Rapid callus induction was optimized by using different concentrations of 2, 4-D (1, 2, 3, 4 and 5 mg/l) or 2, 4-D (2 mg/l) + kn (0.5 mg/l). The results revealed that increased callus weight was significantly different among genotypes and treatments. The value averaged over all the treatments was highest in H65-7052 (1.60 g), followed by LAP (0.93 g) and MOL (0.74 g). MS + 2, 4-D (1-2 mg/l) or MS + 2, 4-D (2 mg/l) + kn (0.5 mg/l) could cause rapid callus growth in all the three sugarcane genotypes. For plant regeneration, all cultured calli on different media exhibited good response to MS. The number of organogenic calli averaged over all the treatments was highest in MOL (61.6), followed by LAP (54.6) and H65-7052 (11.3). The most suitable culture medium for MOL and LAP was MS + 2, 4-D (1 mg/l), while MS + 2,4-D (1 mg/l) or MS + 2,4-D (2 mg/l) + kn (0.5 mg/l) was most appropriate for H65-7052. Moreover, sucrose accumulation in relation to the expression of soluble acid invertase (SAI) gene was measured in calli and plantlets. The highest callus sucrose concentration was found in LAP (141.28 mu mol/g FW), followed by H65-7052 (133.79 mu mol/g FW) and MOL (41.05 mu mol/g FW). The plantlet sucrose concentration showed a similar pattern. The SAI expression in both the callus and plantlet of H65-7052 and LAP was lower than that of MOL. In both systems, sucrose accumulation was negatively correlated with the increase in SAI expression. It may be concluded that callus and plantlet can be used as the model system for investigating sucrose accumulation in sugarcane.

(PDF emailed within 1 workday: $29.90)

Accession: 066309020

Download citation: RISBibTeXText

Related references

Relationship between sucrose accumulation and activities of sucrose-phosphatase, sucrose synthase, neutral invertase and soluble acid invertase in micropropagated sugarcane plants. Acta Physiologiae Plantarum 24(4): 441-446, 2002

Transcript expression and soluble acid invertase activity during sucrose accumulation in sugarcane. Acta Physiologiae Plantarum 33(5): 1749-1757, 2011

Differential expression of soluble acid invertase genes correlates to differences in sucrose accumulation in sugarcane. Plant Physiology (Rockville) 114(3 SUPPL ): 143, 1997

Differential expression of soluble acid invertase genes in the shoots of high-sucrose and low-sucrose species of Saccharum and their hybrids. Australian Journal of Plant Physiology 27(3): 193-199, 2000

Sucrose accumulation in the sugarcane stem is regulated by the difference between the activities of soluble acid invertase and sucrose phosphate synthase. Plant physiology 115(2): 609-616, 1997

Sucrose Accumulation and Internodal Soluble Invertase Isoenzymes in Plant and Ratoon Crops of Sugarcane. Sugar Tech 13(1): 51-59, 2011

Effect of genotype on callus induction and plant regeneration from leaf explants of sugarcane (Saccharum sp.). African Journal of Biotechnology 4(11): 1250-1255, 2005

Structure and expression of soluble acid invertase genes in the stem of high- and low-sucrose accumulating Saccharum species and hybrids. Sugar 2000 Symposium: Sugarcane: research towards efficient and sustainable production: 102-104, 1996

Purification, kinetic and thermodynamic characterization of soluble acid invertase from sugarcane (Saccharum officinarum L.). Plant Physiology and Biochemistry 47(3): 188-194, 2009

Expression analysis of genes associated with sucrose accumulation in sugarcane (Saccharum spp. hybrids) varieties differing in content and time of peak sucrose storage. Plant Biology 17(3): 608-617, 2015

Changes in endogenous hormone content and its relation to sucrose accumulation in sugarcane (Saccharum officinarum L.). Southwest China Journal of Agricultural Sciences 20(3): 392-394, 2007

The response of sugarcane (Saccharum officinarum L) genotypes to callus induction, regeneration and different concentrations of the selective agent (geneticin-418). African Journal of Biotechnology 9(51): 8739-8747, 2010

Internodal soluble invertase isoenzymes in relation to sucrose storage and maturity behaviour in sugarcane cultivars. Sugar Tech 9(1): 43-50, 2007

Sucrose metabolism in the culm of transgenic sugarcane with reduced soluble acid invertase activity. International Society of Sugar Cane Technologists Proceedings of the XXIV Congress, Brisbane, Australia, 17-21 September 2001 Volume 2: 588-591, 2001

Optimization of the Protocols for Callus Induction, Regeneration and ACCLIMATIZATION OF SUGARCANE CV. THATTA-10. Pakistan Journal of Botany 41(2): 815-820, 2009