+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transcriptome Analysis of the Cf-12-Mediated Resistance Response to Cladosporium fulvum in Tomato



Transcriptome Analysis of the Cf-12-Mediated Resistance Response to Cladosporium fulvum in Tomato



Frontiers in Plant Science 7: 2012



Cf-12 is an effective gene for resisting tomato leaf mold disease caused by Cladosporium fulvum (C. fulvum). Unlike many other Cf genes such as Cf-2, Cf-4, Cf-5, and Cf-9, no physiological races of C. fulvum that are virulent to Cf-12 carrying plant lines have been identified. In order to better understand the molecular mechanism of Cf-12 gene resistance response, RNA-Seq was used to analyze the transcriptome changes at three different stages of C. fulvum infection (0, 4, and 8 days post infection [dpi]). A total of 9100 differentially expressed genes (DEGs) between 4 and 0 dpi, 8643 DEGs between 8 and 0 dpi and 2547 DEGs between 8 and 4 dpi were identified. In addition, we found that 736 DEGs shared among the above three groups, suggesting the presence of a common core of DEGs in response to C. fulvum infection. These DEGs were significantly enriched in defense-signaling pathways such as the calcium dependent protein kinases pathway and the jasmonic acid signaling pathway. Additionally, we found that many transcription factor genes were among the DEGs, indicating that transcription factors play an important role in C. fulvum defense response. Our study provides new insight on the molecular mechanism of Cf resistance to C. fulvum, especially the unique features of Cf-12 in responding to C. fulvurn infection.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066326625

Download citation: RISBibTeXText

PMID: 28105042

DOI: 10.3389/fpls.2016.02012


Related references

Differentially expressed gene transcripts related to the Cf-19-mediated resistance response to Cladosporium fulvum infection in tomato. Physiological and Molecular Plant Pathology 89: 8-15, 2015

Physiological and RNA-seq analyses provide insights into the response mechanism of the Cf-10-mediated resistance to Cladosporium fulvum infection in tomato. Plant Molecular Biology 96(4-5): 403-416, 2018

Arbuscular mycorrhiza-mediated resistance in tomato against Cladosporium fulvum-induced mould disease. Journal of Phytopathology 166(1): 67-74, 2018

Studies on breeding tomato for resistance to Cladosporium fulvum. II. On bred strains resistant to C. fulvum. Engei Shikenjo Hokoku/Bull. hort. Res. Sta. : Ser. B Okitsu, 4, 17-32, 1965

Genetic and molecular analysis of tomato Cf genes for resistance to Cladosporium fulvum. Philosophical Transactions of the Royal Society of London. Series B Biological Sciences 353(1374): 1413-1424, 1998

Identification of resistance of tomato source to Cladosporium fulvum and analysis for genetic variation by SSR. 2007

Studies on breeding tomato for resistance to Cladosporium fulvum. I. Breeding material resistant to C. fulvum and the segregation of resistance in early hybrid generations. Engei Shikenjo Hokoku/Bull. hort. Res. Sta., Ser. A. 3, 107-22, 1964

Transcriptome sequencing uncovers the Avr5 avirulence gene of the tomato leaf mold pathogen Cladosporium fulvum. Molecular Plant-Microbe Interactions 27(8): 846-857, 2014

Linkage studies in tomato-Cf genes, determining resistance to tomato leaf mold, Cladosporium fulvum Ck. Canadian Journal of Genetics and Cytology 22(4): 667, 1980

Resistance to Cladosporium fulvum in tomato. Zaadbelangen 29(12): 380, 1975

Natural disulfide bond-disrupted mutants of AVR4 of the tomato pathogen Cladosporium fulvum are sensitive to proteolysis, circumvent Cf-4-mediated resistance, but retain their chitin binding ability. Journal of Biological Chemistry 278(30): 27340-6, 2003

Cladosporium fulvum race 10 and resistance to it in tomato. Can J Bot 42(11): 1555-1558, 1964

Resistance in tomato to new races of cladosporium fulvum. Proceedings of the International Horticultural Congress 1: 125-126, 1970

On the resistance of Tomato varieties to Cladosporium fulvum. Abs. in Phytopathology, 50: 9, 637 p, 1960

Breeding for resistance to Cladosporium fulvum in tomato. Acta Botanica Neerlandica 26(5): 425-426, 1977