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In vitro storage of fish oocytes: effect of storage temperature, media conditions and storage duration on fertilization and larval hatchability of Indian major carp, rohu (Labeo rohita)



In vitro storage of fish oocytes: effect of storage temperature, media conditions and storage duration on fertilization and larval hatchability of Indian major carp, rohu (Labeo rohita)



Aquaculture Research 48(5): 2486-2494



The viability of matured oocytes stored invitro were assessed using carp ovarian fluid (OF) and artificial carp ovarian fluid (ACOF) under different temperature regimes (4, 24, 26, 28 and 30 degrees C) for different storage durations (0, 60, 120, 150 and 180min). Significantly higher fertilization (74%) was achieved when the oocytes were stored using ACOF and 65% in OF after 180min at 28 degrees C. Similarly the hatching rates of larvae were significantly higher in the ACOF and OF, that is, 64% and 47%, respectively, after 180min of storage. The oocytes kept in the storage containers with ACOF having 65% moisture level showed a significantly higher fertilization rate than the 59% moisture level. This study demonstrated that unfertilized matured oocytes (eggs) of rohu can be stored invitro for 180min without compromising the viability (fertilization and hatching) to a great extent in OF and ACOF.

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Accession: 066332418

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DOI: 10.1111/are.13086


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