+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

A novel split-pot bioassay to screen indole acetic acid producing rhizobacteria for the improvement of plant growth in tomato [Solarium lycopersicum L.]



A novel split-pot bioassay to screen indole acetic acid producing rhizobacteria for the improvement of plant growth in tomato [Solarium lycopersicum L.]



Scientia Horticulturae 224: 351-357



In the present study, we developed a split-pot bioassay to screen the indole acetic acid producing rhizobacteria to improve the adventitious root initiation and plant growth promotion in tomato. Based on the ability to produce IAA, 12 isolates were selected among which IRB1 and IRB2 were negative and other rhizobacteria showed a varied level of IAA production (0-84.7 mu g/m1) when grown in media supplemented with increasing concentration of L-tryptophan (0-500 mu g/m1). Results of split-pot bioassay revealed the capability of IAA producing bacteria to stimulate the growth of adventitious roots on tomato stem which was significantly (P <=. 0.05) higher than control and IAA negative bacterial treatments. Further enhanced growth of adventitious roots was in accordance with their IAA producing ability. The probability of obtaining consistent results with split-pot bioassay was analyzed by repeating the experiments ten times and subjecting the data to statistical analysis. The results obtained indicated that split-pot bioassay has significantly lesser variations between the individual experiments in comparison with regular bioassay. Further, randomly selected 31 rhizobacteria with unknown identity and characters from the rhizosphere of tomato plants were screened by employing the split-pot bioassay. Isolates UKR8, UKR19, UKR23 and UKR30 were found significant (P <= 0.05) in increasing the number and length of adventitious roots and increase in root length, shoot length and fresh weight under greenhouse conditions when compared to regular bioassay. It was also recorded that these bacteria were found to produce IAA to various extent. A possible application of split-pot bioassay in screening IAA producing rhizobacteria and their further application in improving tomato plant growth is discussed in the present study.

(PDF emailed within 0-6 h: $19.90)

Accession: 066341301

Download citation: RISBibTeXText

DOI: 10.1016/j.scienta.2017.06.017


Related references

Enzyme activities and growth promotion of spinach by indole-3-acetic acid-producing rhizobacteria. Journal of Horticultural Science and Biotechnology 84(4): 375-380, 2009

Interrelationships of boron deficiency and indole acetic acid oxidase activity in tomato plant (Lycopersicum esculentum Mill). Bulletin of Taiwan Provincial Pingtung Institute of Agriculture 20: 113-119, 1979

Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth. Current Microbiology 62(1): 173-181, 2011

Optimization of Indole Acetic Acid Production by Pseudomonas putida UB1 and its Effect as Plant Growth-Promoting Rhizobacteria on Mustard Brassica nigra. Agricultural Research 2(3): 215-221, 2013

Potential of indole-3-acetic acid-producing rhizobacteria to resist Pb toxicity in polluted soil. Soil and Sediment Contamination 28(1): 101-121, 2019

Rooting in the semihardwood cuttings of olive (Olea europea L.) through the use of exogenous hormones Pakistan, Indole 3-acetic acid, Indole 3-butyric acid, 1-Naphthalene acetic acid, plant growth regulators. Pakistan journal of forestry 31(4): 185-187, 1981

Induced systemic resistance and growth promotion in tomato by an indole-3-acetic acid-producing strain of Paenibacillus polymyxa. Annals of Applied Biology 165(2): 270-279, 2014

Biosynthesis of indole-3-acetic acid in tomato shoots: Measurement, mass-spectral identification and incorporation of (-2)H from (-2)H 2O into indole-3-acetic acid, D- and L-tryptophan, indole-3-pyruvate and tryptamine. Planta 184(3): 368-376, 1991

Influence of auxin producing rhizobacteria on root morphology and nutrient accumulation of crops II Root growth promotion and nutrient accumulation of maize by inoculation with indole-3-acetic acid producing Pseudomonas strains and by exogenously applied IAA under different water supply condition. Angewandte Botanik 69(5-6): 153-156, 1995

Influence of auxin producing rhizobacteria on root morphology and nutrient accumulation of crops. II. Root growth promotion and nutrient accumulation of maize (Zea mays L.) by inoculation with indole-3-acetic acid (IAA) producing Pseudomonas strains and by exogenously applied IAA under different water supply condition. Angewandte Botanik 69(1-2): 37-41, 1995

Preussia sp BSL-10 producing nitric oxide, gibberellins, and indole acetic acid and improving rice plant growth. Journal of Plant Interactions 13(1): 112-118, 2018

First draft genome sequencing of indole acetic acid producing and plant growth promoting fungus Preussia sp. BSL10. Journal of Biotechnology 225: 44-45, 2016

Biosynthesis of indole 3 acetic acid in tomato shoots measurement mass spectral identification and incorporation of deuterium from deuterated water into indole 3 acetic acid d tryptophan l tryptophan indole 3 pyruvate and tryptamine. Planta (Heidelberg) 184(3): 368-376, 1991

Growth stimulation and fruit yield improvement of greenhouse tomato plants by inoculation with Pseudomonas putida or Trichoderma atroviride: Possible role of indole acetic acid (IAA). Soil biology and biochemistry 39(8): 1968-1977, 2007

Indole acetic acid and ACC deaminase fromendophytic bacteria improves the growth of Solanum lycopersicum. Electronic Journal of Biotechnology 21: 58-64, 2016