+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection

MicroRNA profile of immune response in gills of zebrafish (Danio rerio) upon Staphylococcus aureus infection

Fish and Shellfish Immunology 87: 307-314

The gills of fish are large mucosal surfaces that are very important portals for pathogen entry. Investigations have shown that microRNAs (miRNAs) are key regulators of immune response to bacterial infections in the gills of fish; however, how miRNA expression changes in response to infection by Gram-positive bacteria remains largely unknown. To further investigate the immunological role of miRNAs in fish gills under pathogen stress induced by Gram-positive bacterial infection, this study investigated Staphylococcus aureus (SA)-induced changes in the miRNAs levels in gills of adult zebrafish (Danio rerio). miRNA microarrays were used to analyze expression profiles of known miRNA in the gills of zebrafish in response to SA infection and compared these to uninfected control fish. A total of 30 differentially expressed miRNAs (DEMs) were identified. Target genes likely regulated by DEMs were predicted, and functional enrichment analyses were performed. The results indicated that DEM targets were primarily involved in innate immune processes, apoptosis, defense responses, and antibacterial responses. Pathways involving bacterial infection, innate immunity, metabolic process, disease, and apoptosis were mediated by DEMs. Furthermore, real-time quantitative PCR experiments for nine key SA-responsive DEMs that regulated the "SA infection" pathway validated the accuracy of microarray results. Dynamic variations in gene expression were surveyed in detail for these key SA-responsive DEMs for PBS control and at 6, 12, 24, and 48 h after SA challenge in detail. This study provides novel insight into the mechanisms underlying the miRNA regulation during the SA-induced immune response in zebrafish gills, and provides basic knowledge on the innate immune response against Gram-positive bacterial infection in bony fish.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 066444588

Download citation: RISBibTeXText

PMID: 30677514

DOI: 10.1016/j.fsi.2019.01.026

Related references

Immune response in the skin of zebrafish, Danio rerio (Hamilton), against Staphylococcus chromogenes. Journal of Fish Diseases 35(9): 699-704, 2012

Zebrafish Danio rerio as a model to study the immune response against infection with Ichthyophthirius multifiliis. Journal of Fish Diseases 40(6): 847-852, 2017

A zebrafish (Danio rerio) bloodthirsty member 20 with E3 ubiquitin ligase activity involved in immune response against bacterial infection. Biochemical and Biophysical Research Communications 457(1): 83-89, 2015

Skin immune response in the zebrafish, Danio rerio (Hamilton), to Aeromonas hydrophila infection: a transcriptional profiling approach. Journal of Fish Diseases 38(2): 137-150, 2015

Microplastic accumulation patterns and transfer of benzo[a]pyrene to adult zebrafish (Danio rerio) gills and zebrafish embryos. Environmental Pollution 235: 918-930, 2018

Immune response of zebrafish (Danio rerio) against a newly isolated bacterial pathogen Aeromonas hydrophila. Fish and Shellfish Immunology 25(3): 239-249, 2008

Innate pro-inflammatory immune response and granuloma formation in the kidney of adult zebrafish (Danio rerio). Fish & Shellfish Immunology 34(6): 1712-1713, 2013

Analysis of zebrafish (Danio rerio) behavior in response to bacterial infection using a self-organizing map. Bmc Veterinary Research 11: 269, 2015

Morphofunctional Alterations in Zebrafish (Danio rerio) Gills after Exposure to Mercury Chloride. International Journal of Molecular Sciences 18(4):, 2017

Bacterial lipopolysaccharides induce genes involved in the innate immune response in embryos of the zebrafish (Danio rerio). Fish and Shellfish Immunology 23(4): 901-905, 2007

Neutrophil migration in the activation of the innate immune response to different Flavobacterium psychrophilum vaccines in zebrafish (Danio rerio). Journal of Immunology Research 2015: 515187, 2015

Pseudocapillaria tomentosa in laboratory zebrafish Danio rerio: patterns of infection and dose response. Diseases of Aquatic Organisms 131(2): 121-131, 2018

Effects of arsenic (As) exposure on the antioxidant status of gills of the zebrafish Danio rerio (Cyprinidae). Comparative Biochemistry and Physiology. Toxicology and Pharmacology 149(4): 538-543, 2009

Effect of Dietary N -acyl Homoserin Lactonase on the Immune Response and the Gut Microbiota of Zebrafish, Danio rerio , Infected with Aeromonas hydrophila. Journal of the World Aquaculture Society 45(2): 149-162, 2014

Protective Yeasts Control V. anguillarum Pathogenicity and Modulate the Innate Immune Response of Challenged Zebrafish ( Danio rerio ) Larvae. Frontiers in Cellular and Infection Microbiology 6: 127, 2016