+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

First Report of Septoria apocyni Causing Spot Blight on the Species of Apocynum venetum and Poacynum pictum in China



First Report of Septoria apocyni Causing Spot Blight on the Species of Apocynum venetum and Poacynum pictum in China



Plant Disease 98(10): 1429



The species of Apocynum venetum and Poacynum pictum grow widely from the middle to northwestern regions of China. During the summers of 2011 to 2013, a spot blight was found in wild and cultivated both species in Altay Prefecture of the Xinjiang Uygur Autonomous Region, China. The spot blight caused leaf yellowing and leaf drop, and serious damage to plant phloem. Lesions were circular to irregular, and the diameter of lesions on A. venetum and P. pictum was 1.84 to 6.84 × 1.23 to 4.24 mm and 2.05 to 7.09 × 1.46 to 5.65 mm, respectively. Pycnidia were 70 to 115 × 52 to 120 μm, scattered, spherical, buried, and had a brown hard shell with a prominent ostiole. Conidia were colorless, needle-shaped, or linear. The conidia base was obtuse, containing 3 to 5 indistinct septa, 46.3 to 110.3 × 2 to 2.5 μm. Fungal cultures were obtained by cutting 1-cm-long infected leaf pieces from the margins of the lesions following routine surface sterilizing procedures. The sections were placed on potato dextrose agar (PDA) in petri dishes and incubated at 23°C for 4 weeks (4). Hyphae had septa, the aerial and base mycelium was white and rufous, and the back of the colony was sunken and cracked after 2 weeks, but no spore was observed. To verify the identity, total DNA was extracted directly from fungal mycelium with a UNIQ-10 fungal genomic DNA extraction kit (Sangon Biotech, Shanghai, China) and PCR amplification performed with primers ITS1/ITS4 (3). A 512-bp PCR product was sequenced and contrasted with GenBank sequences using BLAST, which revealed 99% identity with Septoria sp. (GenBank Accession No. KC134322.1). To confirm pathogenicity, A. venetum and P. pictum were planted in pots and grown in a greenhouse. After 6 weeks of growth, plants were inoculated by spraying a mycelial suspension onto the foliage while control plants received a similar application of sterilized distilled water. Five pots (3 plants per pot) were used for each treatment. The pots were then placed on plates filled with tap water and covered with Plexiglas hoods in the greenhouse at 20 to 25°C. Lesions began to appear 6 to 7 days after inoculation with the mycelial suspension, whereas control plants remained healthy. The average disease incidence was 19.3%. The symptoms and morphology were similar to Septoria apocyni in Teterevnikova (2). It was determined that spot blight of A. venetum and P. pictum was caused by S. apocyni based on morphological comparison. There is one relevant literature report of spot blight on A. venetum and P. pictum in China, but without any details of the pathogenicity or morphology of the pathogen (1). We believe that this is the first report of S. apocyni occurring on the species of A. venetum and P. pictum in China. References: (1) W. Sun et al. Special Economic Animal and Plant 8:23, 2005. (2) D. N. Teterevnikova. Page 79 in: Septoria sp. Fungus of USSR. Armenian Academy of Sciences Publishing, Armenia, USSR, 1987. (3) G. J. M. Verkley et al. Mycologia 96:558, 2004. (4) W. Zhang et al. Plant Dis. 96:1374, 2012.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 066446129

Download citation: RISBibTeXText

PMID: 30704000


Related references

Factors Influencing Rust (Melampsora apocyni) Intensity on Cultivated and Wild Apocynum venetum in Altay Prefecture, China. Phytopathology 2018, 2018

Apocynum venetum L. and Apocynum pictum Schrenk (Apocynaceae) as multi-functional and multi-service plant species in Central Asia: a review on biology, ecology, and utilization. Journal of Applied Botany and Food Quality 85(2): 159-167, 2012

Micromorphological characters of the seeds of Apocynum venetum and Poacynum hendersonii. Xibei Zhiwu Xuebao 20(3): 476-479, 2000

The anatomical structure of the roots and rhizomes of Trachomitum venetum and Poacynum pictum growing in Kazakhstan. Rastitel' nye Resursy 12(3): 372-380, 1976

Anatomical structure of the root and rhizome of Trachomitum venetum and Poacynum pictum growing in Karzakhstan. Rastitel'nye resursy: 2 (3) 372-380, 1976

Seasonal, spatial, and interspecific variation in quercetin in Apocynum venetum and Poacynum hendersonii, Chinese traditional herbal teas. Journal of Agricultural and Food Chemistry 51(8): 2390-2393, 2003

Comparison of the constituents of Apocynum venetum and acidified Apocynum venetum by liquid chromatography–UV diode array detection–electrospray ionisation mass spectrometry. Medicinal Chemistry Research 21(8): 1684-1691, 2012

The influence of irrigation frequency on the occurrence of rust disease (Melampsora apocyni) and determination of the optimum irrigation regime in organic Apocynum venetum production. Agricultural Water Management 205: 81-89, 2018

Discrimination between Leave of Apocynum venetum and Its Adulterant, A. pictum Based on Antioxidant Assay and Chemical Profiles Combined with Multivariate Statistical Analysis. Antioxidants 4(2): 359-372, 2016

Studies on septori-oses of plants. II. Septoria azaleae Voglino causing the brown-spot disease of the cultivated azaleas in Japan, by TAKEWO HEMMI and SHIZUKO KURATA. III. On Septoria callistephi Gloyer pathogenic on the China aster, by HISAO NAKAMURA. IV. New or noteworthy species of Septoria found in Japan, by SHIGEKATSU HIRAYAMA. Mem Coll Agric Kyoto Imp Univ 13: 1-22, 23-31, 33-40, 1931

Development of randomly amplified polymorphic DNA-sequence characterized amplified region marker for identification of Apocynum venetum LINN. from A. pictum SCHRENK. Biological & Pharmaceutical Bulletin 33(3): 522-526, 2010

Pharmacognostical studies of Apocynum (2): Comparative anatomy of the leaves of Apocynum and Poacynum spp. Shoyakugaku Zasshi 47(4): 388-395, 1994

Albinism and mosaicism in Apocynum venetum associated with viral infections in China. Archives of Virology: -, 2018

Quercetin, isoquercitrin, and apocynin from Apocynum lancifolium and Apocynum pictum. Chemistry of natural compounds: (Transl 1975), 9 (3) 404, 1973

Quercetin iso quercitrin and apocynine from apocynum lancifolium and apocynum pictum. Khimiya Prirodnykh Soedinenii 9(2): 431, 1973