+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Integrated Microfluidic Devices Fabricated in Poly (Methyl Methacrylate) (PMMA) for On-site Therapeutic Drug Monitoring of Aminoglycosides in Whole Blood



Integrated Microfluidic Devices Fabricated in Poly (Methyl Methacrylate) (PMMA) for On-site Therapeutic Drug Monitoring of Aminoglycosides in Whole Blood



Biosensors 9(1)



On-site therapeutic drug monitoring (TDM) is important for providing a quick and accurate dosing to patients in order to improve efficacy and minimize toxicity. Aminoglycosides such as amikacin, gentamicin, and tobramycin are important antibiotics that have been commonly used to treat infections of chronic bacterial infections in the urinary tract, lung, and heart. However, these aminoglycosides can lead to vestibular and auditory dysfunction. Therefore, TDM of aminoglycosides is important due to their ototoxicity and nephrotoxicity. Here, we have developed a hot embossed poly (methyl methacrylate) (PMMA) microfluidic device featuring an electrokinetic size and mobility trap (SMT) to purify, concentrate, and separate the aminoglycoside antibiotic drugs amikacin, gentamicin, and tobramycin. These drugs were separated successfully from whole blood within 3 min, with 30-fold lower detection limits compared to a standard pinched injection. The limit of detections (LOD) were 3.75 µg/mL for gentamicin, 8.53 µg/mL for amikacin, and 6.00 µg/mL for tobramycin. These are sufficient to cover the therapeutic range for treating sepsis of 6⁻10 μg/mL gentamicin and tobramycin and 12⁻20 μg/mL of amikacin. The device is simple and could be mass produced via embossing or injection molding approaches.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066446185

Download citation: RISBibTeXText

PMID: 30704056

DOI: 10.3390/bios9010019


Related references

Microfluidic devices fabricated in poly(methyl methacrylate) using hot-embossing with integrated sampling capillary and fiber optics for fluorescence detection. Lab on a Chip 2(2): 88-95, 2004

Thermal assisted ultrasonic bonding method for poly(methyl methacrylate) (PMMA) microfluidic devices. Talanta 81(4-5): 1331-1338, 2010

Fabrications of a poly (methyl methacrylate) (PMMA) microfluidic chip-based DNA analysis device. Guang Pu Xue Yu Guang Pu Fen Xi 29(12): 3379-3382, 2014

Carbon nanotube/poly(methyl methacrylate) (CNT/PMMA) composite electrode fabricated by in situ polymerization for microchip capillary electrophoresis. Chemistry 13(3): 846-853, 2006

Microarrays assembled in microfluidic chips fabricated from poly(methyl methacrylate) for the detection of low-abundant DNA mutations. Analytical Chemistry 75(5): 1130-1140, 2003

Simplified immobilisation method for histidine-tagged enzymes in poly(methyl methacrylate) microfluidic devices. New Biotechnology 47: 31-38, 2017

Surface modification of poly(methyl methacrylate) microfluidic devices for high-resolution separations of single-stranded DNA. Electrophoresis 28(6): 984-993, 2007

Contact conductivity detection in poly(methyl methacrylate)-based microfluidic devices for analysis of mono- and polyanionic molecules. Analytical Chemistry 74(10): 2407-2415, 2002

Electrical bistabilities and memory mechanisms of organic bistable devices fabricated utilizing SnO2 nanoparticles embedded in a poly(methyl methacrylate) layer. Journal of Nanoscience and Nanotechnology 10(11): 7735-7738, 2010

Cationic starch derivatives as dynamic coating additives for analysis of amino acids and peptides using poly(methyl methacrylate) microfluidic devices. Analytical Chemistry 76(22): 6792-6796, 2004

pH-sensitive micelles self-assembled from multi-arm star triblock co-polymers poly(ε-caprolactone)-b-poly(2-(diethylamino)ethyl methacrylate)-b-poly(poly(ethylene glycol) methyl ether methacrylate) for controlled anticancer drug delivery. Acta Biomaterialia 9(8): 7679-7690, 2013

Development of micro-flow devices by direct-milling on poly(methyl methacrylate) substrates with integrated optical detection. Microchimica Acta 166(3-4): 189-195, 2009

Self-assembly between graphene sheets and cationic poly(methyl methacrylate) (PMMA) particles: preparation and characterization of PMMA/graphene composites. Journal of Nanoparticle Research 14(1): 717-0, 2012

On the degradation kinetics of poly(ethylene terephthalate) (PET)/poly(methyl methacrylate) (PMMA) blends in dynamic thermogravimetry. Polymer Degradation and Stability 104: 28-32, 2014

Acrylic AB and ABA block copolymers based on poly(2-ethylhexyl acrylate) (PEHA) and poly(methyl methacrylate) (PMMA) via ATRP. Acs Applied Materials and Interfaces 4(8): 4200-4207, 2012