+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress



Heat Shock Protein 27 Phosphorylation Regulates Tumor Cell Migration under Shear Stress



Biomolecules 9(2)



Heat shock protein 27 (HSP27) is a multifunctional protein that undergoes significant changes in its expression and phosphorylation in response to shear stress stimuli, suggesting that it may be involved in mechanotransduction. However, the mechanism of HSP27 affecting tumor cell migration under shear stress is still not clear. In this study, HSP27-enhanced cyan fluorescent protein (ECFP) and HSP27-Ypet plasmids are constructed to visualize the self-polymerization of HSP27 in living cells based on fluorescence resonance energy transfer technology. The results show that shear stress induces polar distribution of HSP27 to regulate the dynamic structure at the cell leading edge. Shear stress also promotes HSP27 depolymerization to small molecules and then regulates polar actin accumulation and focal adhesion kinase (FAK) polar activation, which further promotes tumor cell migration. This study suggests that HSP27 plays an important role in the regulation of shear stress-induced HeLa cell migration, and it also provides a theoretical basis for HSP27 as a potential drug target for metastasis.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066446243

Download citation: RISBibTeXText

PMID: 30704117

DOI: 10.3390/biom9020050


Related references

Heat shock protein 27 kDa expression and phosphorylation regulates endothelial cell migration. Faseb Journal 12(14): 1481-1490, 1998

Protein kinase A -mediated phosphorylation regulates heat shock protein 60 cell surface expression on human T cells. FASEB Journal 16(4): A318, 2002

Endoplasmic Reticulum-resident Heat Shock Protein 90 (HSP90) Isoform Glucose-regulated Protein 94 (GRP94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport. Journal of Biological Chemistry 291(16): 8309-8323, 2016

The mitogen-activated protein kinase Slt2 regulates nuclear retention of non-heat shock mRNAs during heat shock-induced stress. Molecular and Cellular Biology 30(21): 5168-5179, 2010

Heat shock protein 70-binding protein 1 is highly expressed in high-grade gliomas, interacts with multiple heat shock protein 70 family members, and specifically binds brain tumor cell surfaces. Cancer Science 100(10): 1870-1879, 2009

Heat shock protein 25 or inducible heat shock protein 70 activates heat shock factor 1: dephosphorylation on serine 307 through inhibition of ERK1/2 phosphorylation. Journal of Biological Chemistry 281(25): 17220-7, 2006

Fluid shear stress induces the phosphorylation of small heat shock proteins in vascular endothelial cells. American Journal of Physiology 271(3 Pt 1): C994-1000, 1996

New insight into the signalling pathways of heat stress-induced myocardial preconditioning: protein kinase Cepsilon translocation and heat shock protein 27 phosphorylation. Clinical and Experimental Pharmacology and Physiology 31(3): 129-133, 2004

Induction of Heat Shock Protein 70 as a Predictive Marker of the Tumor Cell Radiosensitization with Inhibitors of the Heat Shock Protein 90 Activity. Radiatsionnaia Biologiia, Radioecologiia 56(5): 494-502, 2016

Protein kinase C delta regulates the phosphorylation of heat shock protein 27 in human hepatocellular carcinoma. Life Sciences 81(7): 585-591, 2007

The heat shock stress response after brain lesions: induction of 72 kDa heat shock protein (cell types involved, axonal transport, transcriptional regulation) and protein synthesis inhibition. Progress in Neurobiology 51(6): 607-636, 1997

Cell-free phosphorylation of the murine small heat-shock protein hsp25 by an endogenous kinase from Ehrlich ascites tumor cells. Biochimica et Biophysica Acta 1136(2): 203-207, 1992

Altered temporal profile of heat shock factor 1 phosphorylation and heat shock protein 70 expression induced by heat shock in nucleus tractus solitarii of spontaneously hypertensive rats. Circulation 107(2): 339-345, 2003

Upregulation of heat shock protein 70 and the differential protein expression induced by tumor necrosis factor-alpha enhances migration and inhibits apoptosis of hepatocellular carcinoma cell HepG2. International Journal of Medical Sciences 14(3): 284-293, 2017

Alpha2 adrenoreceptor agonist regulates protein kinase C-induced heat shock protein 27 phosphorylation in C6 glioma cells. Journal of Neurochemistry 106(2): 519-528, 2008