+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Transthyretin Amyloid Fibril Disrupting Activities of Extracts and Fractions from Juglans mandshurica Maxim. var. cordiformis (Makino) Kitam

Transthyretin Amyloid Fibril Disrupting Activities of Extracts and Fractions from Juglans mandshurica Maxim. var. cordiformis (Makino) Kitam

Molecules 24(3)

Transthyretin-related amyloidosis is a slowly progressive disorder caused by deposition of insoluble amyloid plaques formed by fibrillization of mutant or defective transthyretin (TTR) monomers that leads to neurodegeneration and organ failure. Thus, any compound exhibiting TTR amyloid formation inhibitory activity or TTR amyloid fibril disrupting activity might be a potential candidate for the development of therapies for these disorders. Our aim in this study was the evaluation of the TTR amyloid fibril disrupting potential of extracts of leaves and immature fruits of two Juglans plants, i.e., Juglans mandshurica var. sachalinensis and Juglans mandshurica var. cordiformis. The TTR amyloid fibril disrupting activity was measured by Thioflavin-T (ThT) assay and PROTEOSTAT® Protein aggregation assay methods. A fifty percent acetone extract of the fruits of Juglans mandshurica var. cordiformis showed strong amyloid fibril disrupting activity, and was further fractionated using different solvents. Ethyl acetate and n-butanol fractions showed significant activity in both assays. Syringic acid was isolated and identified as main compound in both of these fractions; however, it did not show any activity. Furthermore, some of the previously reported compounds from Juglans plants including naphthoquinone derivatives and phenolic compounds were evaluated to identify the potential bioactive compounds. Among them, juglone, a naphthoquinone derivative showed promising activity. However, juglone also showed strong cytotoxicity in HEK293 cells. Thus, future studies should focus on the isolation and identification of naphthoquinone derivatives or other compounds from Juglans plan ts with potent bioactivity and low cytotoxicity.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 066446246

Download citation: RISBibTeXText

PMID: 30704121

DOI: 10.3390/molecules24030500

Related references

Insecticidal activities of alcoholic extracts from bark of Juglans mandshurica Maxim. Journal of Northeast Forestry University 35(1): 60-62, 2007

Studies on the constituents of Juglans species. I. Structural determination of (4S)- and (4R)-4-hydroxy-alpha-tetralone derivatives from the fruit of Juglans mandshurica MAXIM. var. sieboldiana MAKINO. Chemical & Pharmaceutical Bulletin 53(8): 934-937, 2005

A new phenolic glycoside syringate from the bark of Juglans mandshurica MAXIM. var. sieboldiana MAKINO. Journal of Natural Medicines 63(2): 220-222, 2009

Juglans mandshurica Maxim extracts exhibit antitumor activity on HeLa cells in vitro. Molecular Medicine Reports 9(4): 1313-1318, 2014

Chemical constituents and antioxidant activities of the extractives from Juglans mandshurica Maxim bark. Chemistry And Industry Of Forest Products: 1, 29-32, 2008

Effects of Juglans mandshurica maxim extracts on Toxoplasma gondii swallowed function of peritoneal macrophage in mice. Zhongguo Bingyuan Shengwuxue Zazhi: 8, 591-592, 597, 2008

α-Tetralone glycosides from the green walnut husks of Juglans mandshurica Maxim. and their cytotoxic activities. Natural Product Research 2019: 1-9, 2019

Protein Hydrolyzates from Changbai Mountain Walnut ( Juglans mandshurica Maxim.) Boost Mouse Immune System and Exhibit Immunoregulatory Activities. Evidence-Based Complementary and Alternative Medicine 2018: 4576561, 2018

Suppression of soil acidification by the stem flows of some tree species. (II.) The cases of Kalopanax pictus (Thunb.) Nakai and Juglans mandshurica Maxim. subsp. sieboldiana (Maxim.) Kitamura. Journal of the Japanese Forestry Society 75(4): 321-330, 1993

The anti-tumor effect and biological activities of the extract JMM6 from the stem-barks of the Chinese Juglans mandshurica Maxim on human hepatoma cell line BEL-7402. African Journal of Traditional, Complementary, and Alternative Medicines 10(2): 258-269, 2014

Chemical constituents from the roots of Juglans mandshurica Maxim. Chinese Journal Of Natural Medicines: 5, 354-356, 2008

Extraction technology of total flavone in Juglans mandshurica Maxim. Journal of Northeast Forestry University 34(1): 38-39, 2006

Polyphenolic profiles and antioxidant activities of heartnut (Juglans ailanthifolia Var. cordiformis) and Persian walnut (Juglans regia L.). Journal of Agricultural and Food Chemistry 54(21): 8033-8040, 2006

A new naphthalenone isolated from the green walnut husks of Juglans mandshurica Maxim. Natural Product Research 29(2): 174-179, 2015

Extraction and determination of total flavonoid and juglone in Juglans mandshurica Maxim. Chemistry and Industry of Forest Products 26(2): 93-95, 2006