+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Value of the detection of BRAF (V600E) gene mutation and protein expression in auxiliary cytological diagnosis of papillary thyroid carcinoma

Value of the detection of BRAF (V600E) gene mutation and protein expression in auxiliary cytological diagnosis of papillary thyroid carcinoma

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 54(1): 18-22

目的: 评价BRAF(V600E)基因检测对不同诊断级别的甲状腺细针穿刺(fine needle aspiration,FNA)病例辅助诊断的意义;探讨甲状腺FNA液基标本中BRAF蛋白(VE1)免疫细胞化学能否作为识别BRAF(V600E)基因突变的方法。 方法: 收集在中国医学科学院肿瘤医院行甲状腺FNA检查,并有剩余细胞学液基标本的病例273例,应用聚合酶链反应进行BRAF(V600E)基因检测,同时对其中78例细胞量充足的病例应用免疫细胞化学的方法进行VE1蛋白的检测,并与临床病理结果进行对比分析。使用SPSS17.0对数据进行统计分析。 结果: 在273例FNA病例中,细胞学明确诊断203例,不明确70例。联合BRAF(V600E)检测,可以使9例诊断为意义不明的非典型细胞中的3例(3/9)和46例可疑甲状腺乳头状癌中的31例(67.4%)确诊为甲状腺乳头状癌。VE1蛋白表达的灵敏度为62.8%(27/43),特异度为91.4%(32/35)。与BRAF(V600E)基因有中等程度的一致性(κ=0.524,P<0.05)。 结论: 对细胞学诊断为意义不明的非典型细胞和可疑癌病例联合BRAF(V600E)检测可以增加甲状腺乳头状癌的诊断率;对于无法进行基因检测的病例,VE1免疫细胞化学可以作为识别BRAF(V600E)突变的方法。.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 066446289

Download citation: RISBibTeXText

PMID: 30704164

Related references

Clinical Manifestations and Gene Expression in Patients with Conventional Papillary Thyroid Carcinoma Carrying the BRAF(V600E) Mutation and BRAF Pseudogene. Thyroid 26(5): 691-704, 2016

BRAF V600E detection in cytological thyroid samples: A key component of the decision tree for surgical treatment of papillary thyroid carcinoma. Head and Neck 38(7): 1017-1021, 2016

Molecular detection of the BRAF (V600E) activating gene mutation in fine needle aspiration of papillary thyroid carcinoma using LightCycler PCR and fluorescent melting curve analysis. 2005

Clinical utility of immunohistochemistry for the detection of the BRAF v600e mutation in papillary thyroid carcinoma. Surgery 154(6): 1199-204; Discussion 1204-5, 2013

Effects of BRAF(V600E) mutation on Na(+)/I(-) symporter expression in papillary thyroid carcinoma. Journal of Huazhong University of Science and Technology. Medical Sciences 36(1): 77-81, 2016

BRAF(V600E) mutation analysis from May-Grünwald Giemsa-stained cytological samples as an adjunct in identification of high-risk papillary thyroid carcinoma. Endocrine Pathology 22(4): 195-199, 2011

Analysis of clinicopathological characteristics of papillary thyroid carcinoma and BRAF V600E gene mutation. Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 32(19): 1472-1476, 2018

Advances in the research of BRAF(V600E) gene mutation correlated with papillary thyroid carcinoma. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 51(8): 630-634, 2016

Specific immunohistochemical detection of the BRAF V600E mutation in primary and metastatic papillary thyroid carcinoma. Experimental and Molecular Pathology 100(1): 236-241, 2016

BRAF V600E mutation in papillary thyroid carcinoma: prevalence and detection in fine needle aspiration specimens. Annales de Pathologie 30(4): 252-262, 2010

Increased CYP24A1 expression is associated with BRAF(V600E) mutation and advanced stages in papillary thyroid carcinoma. Clinical Endocrinology 81(1): 109-116, 2014

Efficient detection of the V600E mutation of the BRAF gene in papillary thyroid carcinoma using multiplex allele-specific polymerase chain reaction combined with denaturing high-performance liquid chromatography. Genetics and Molecular Research 12(4): 4990-4997, 2013

A Case of Multifocal Papillary Thyroid Carcinoma Consisting of One Encapsulated Follicular Variant with BRAF K601E Mutation and Three Conventional Types with BRAF V600E Mutation. Korean Journal of Pathology 47(3): 293-298, 2013

Detection of BRAF V600E mutation in fine-needle aspiration fluid of papillary thyroid carcinoma by droplet digital PCR. Clinica Chimica Acta; International Journal of Clinical Chemistry 491: 91-96, 2019

Diagnostic value of immunohistochemistry for the detection of the BRAF(V600E) mutation in papillary thyroid carcinoma: comparative analysis with three DNA-based assays. Thyroid 24(5): 858-866, 2014