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The impact of meisoindigo on apoptosis and proliferation of SET2 cell line by JAK-STAT pathway



The impact of meisoindigo on apoptosis and proliferation of SET2 cell line by JAK-STAT pathway



Zhonghua Xue Ye Xue Za Zhi 40(1): 29-34



目的: 观察甲异靛对JAK2/V617F杂合突变细胞系SET2细胞凋亡及增殖的影响,并探讨JAK-STAT信号通路在其中所起的作用。 方法: 不同浓度甲异靛(0、5、10 μmol/L)作用于SET2细胞24、48、72 h后,采用流式细胞术检测细胞凋亡。不同浓度甲异靛(0、5、10、20 μmol/L)作用于SET2细胞24、48、72、96 h后,CCK8法检测细胞增殖。不同浓度甲异靛(0、5、10、20 μmol/L)作用于SET2细胞12 h后,在甲基纤维素半固体培养基进行集落培养,Western blot法测定JAK-STAT信号通路相关蛋白及凋亡相关蛋白表达水平。 结果: 在0、5、10 μmol/L甲异靛作用于SET2细胞后24、48和72 h,细胞凋亡率均随药物浓度的增大而增高(P<0.01)。0、5、10、20 μmol/L甲异靛作用SET2细胞后,随着药物浓度增加,细胞增殖受抑制(P<0.01)。不同浓度甲异靛处理SET2细胞后,其集落形成率随药物浓度增加而降低[0 μmol/L甲异靛组为(4.48±1.19)%,20 μmol/L甲异靛组为(2.55±0.36)%,Dunnett P=0.020]。不同浓度甲异靛作用于SET2细胞12 h后,JAK2、P-JAK2、P-STAT1、P-STAT3、P-STAT3和STAT5的表达水平均有随药物浓度增加而逐渐下调的趋势,抗凋亡蛋白BCL-2、BCL-XL的表达水平随药物浓度增加而下调,促凋亡蛋白BID、BIM的表达水平随药物浓度增加而上调。 结论: 甲异靛对Ph阴性骨髓增殖性肿瘤细胞系SET2细胞具有明显的促进凋亡、抑制增殖作用,伴随着抗凋亡蛋白表达下调及促凋亡蛋白表达上调,其作用机制可能与甲异靛抑制JAK-STAT信号通路有关。.

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Accession: 066446343

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PMID: 30704225


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