+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Conserved regulation of RNA processing in somatic cell reprogramming

Conserved regulation of RNA processing in somatic cell reprogramming

Bmc Genomics 20(1): 100

Along with the reorganization of epigenetic and transcriptional networks, somatic cell reprogramming brings about numerous changes at the level of RNA processing. These include the expression of specific transcript isoforms and 3' untranslated regions. A number of studies have uncovered RNA processing factors that modulate the efficiency of the reprogramming process. However, a comprehensive evaluation of the involvement of RNA processing factors in the reprogramming of somatic mammalian cells is lacking. Here, we used data from a large number of studies carried out in three mammalian species, mouse, chimpanzee and human, to uncover consistent changes in gene expression upon reprogramming of somatic cells. We found that a core set of nine splicing factors have consistent changes across the majority of data sets in all three species. Most striking among these are ESRP1 and ESRP2, which accelerate and enhance the efficiency of somatic cell reprogramming by promoting isoform expression changes associated with mesenchymal-to-epithelial transition. We further identify genes and processes in which splicing changes are observed in both human and mouse. Our results provide a general resource for gene expression and splicing changes that take place during somatic cell reprogramming. Furthermore, they support the concept that splicing factors with evolutionarily conserved, cell type-specific expression can modulate the efficiency of the process by reinforcing intermediate states resembling the cell types in which these factors are normally expressed.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 066446506

Download citation: RISBibTeXText

PMID: 30704403

DOI: 10.1186/s12864-019-5438-2

Related references

Epigenetic regulation of somatic cell reprogramming. Current Opinion in Genetics and Development 46: 156-163, 2017

Regulation of somatic cell reprogramming through inducible mir-302 expression. Nucleic Acids Research 39(3): 1054-1065, 2011

Regulation of L-threonine dehydrogenase in somatic cell reprogramming. Stem Cells 31(5): 953-965, 2014

Alternative splicing regulation during somatic cell reprogramming. Seikagaku. Journal of Japanese Biochemical Society 86(6): 807-811, 2015

Shared gene regulation during human somatic cell reprogramming. Journal of Genetics and Genomics 39(12): 613-623, 2013

Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming. Stem Cell Reports 4(6): 1125-1139, 2016

The function and regulation of mesenchymal-to-epithelial transition in somatic cell reprogramming. Current Opinion in Genetics and Development 28: 32-37, 2015

Jak/Stat3 signaling promotes somatic cell reprogramming by epigenetic regulation. Stem Cells 30(12): 2645-2656, 2013

Regulation of the DNA Methylation Landscape in Human Somatic Cell Reprogramming by the miR-29 Family. Stem Cell Reports 7(1): 43-54, 2017

Multifaceted regulation of somatic cell reprogramming by mRNA translational control. Cell Stem Cell 14(5): 606-616, 2014

MicroRNAs in regulation of pluripotency and somatic cell reprogramming: small molecule with big impact. Rna Biology 10(8): 1255-1261, 2014

MicroRNA-mediated regulation of extracellular matrix formation modulates somatic cell reprogramming. Rna 20(12): 1900-1915, 2015

Down-Regulation of H3K4me3 by MM-102 Facilitates Epigenetic Reprogramming of Porcine Somatic Cell Nuclear Transfer Embryos. Cellular Physiology and Biochemistry 45(4): 1529-1540, 2018

Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing. Bmc Genomics 19(1): 734, 2018

Bright/Arid3A acts as a barrier to somatic cell reprogramming through direct regulation of Oct4, Sox2, and Nanog. Stem Cell Reports 2(1): 26-35, 2014