+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus



Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus



Microbial Cell Factories 18(1): 21



Infectious bursal disease (IBD) is an acute contagious immunosuppressive disease which lead to acute bursal injury and immune dysfunction in poultry. It has caused heavy economic losses in the commercial poultry industry for many years in worldwide. Attenuated live vaccine has widely used in poultry showing some promising signs against IBDV infection. But it has defects such as generating enhanced virulence and immunosuppression prohibits. Therefore, the development of mucosal vaccines using the food-grade lactic acid bacterium is necessary. Here, we construct a recombinant Lactococcus co-expressing the major IBDV antigens VP2 and RCK protein of Salmonella enterica to prevent IBD. The recombinant fusion protein VP2-RCK was expressed in a soluble and stable form in the cytoplasm of the recombinant Lactococcus lactis. Animal experiments showed that: (1) the survival rates of the injected immunization inactivated recombinant LAB group and oral immunization live recombinant LAB group were 100% and 80%, respectively; (2) ELISA titers of all serum samples from all experimental groups were negative, but high amounts of specific neutralizing antibodies were detected (1:210 to 1:212); and (3) the bursas of the injected immunization inactivated recombinant LAB group did not suffer damage, as confirmed by clinical observation and bursal histopathological examination. Our results indicate that r-L. lactis-OptiVP2-RCK induces a specific neutralizing-antibody-mediated immune response that confers full protection against very-virulent IBDV (vvIBDV) challenge. Lactococcus lactis NZ3900 strain and its matching plasmid pNZ8149 could express the recombinant fusion protein VP2-RCK in a soluble form in the cytoplasm. The protective efficacy of r-L. lactis-OptiVP2-RCK (100%) was better than r-L. lactis-OptiVP2 (0%) which prove RCK protein played its unique role. The neutralizing antibodies titers against infectious bursal disease virus via one-time vaccination with inactivated r-L. lactis-OptiVP2-RCK could reach 1:210 to 1:212, but ELISA titers of all serum samples were negative. For this phenomenon, perhaps because of the change of delivery pathway or the spatial structure of fusion protein. We need further study to test these hypotheses.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066446593

Download citation: RISBibTeXText

PMID: 30704494

DOI: 10.1186/s12934-019-1061-9


Related references

Recombinant infectious bursal disease virus expressing Newcastle disease virus (NDV) neutralizing epitope confers partial protection against virulent NDV challenge in chickens. Antiviral Research 101: 1-11, 2014

Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens. Virologica Sinica 31(3): 266-269, 2017

Avian adenovirus CELO recombinants expressing VP2 of infectious bursal disease virus induce protection against bursal disease in chickens. Vaccine 22(17-18): 2351-2360, 2004

Avian adenovirus Celo recombinants expressing Vp2 of infectious bursal disease virus induce protection against bursal disease in chickens. Vaccine 22(17-18): 2351-2360, 2004

A recombinant turkey herpesvirus expressing chicken interleukin-2 increases the protection provided by in ovo vaccination with infectious bursal disease and infectious bronchitis virus. Vaccine 25(51): 8529-8535, 2007

Immunogenicity of recombinant Lactobacillus casei expressing VP2 protein of infectious bursal disease virus in chickens. Sheng Wu Gong Cheng Xue Bao 30(11): 1679-1690, 2015

Baculovirus virions displaying infectious bursal disease virus VP2 protein protect chickens against infectious bursal disease virus infection. Avian Diseases 55(2): 223-229, 2011

Protective vaccination against infectious bursal disease virus with whole recombinant Kluyveromyces lactis yeast expressing the viral VP2 subunit. Plos One 7(9): E42870, 2013

Multivalent inactivated virus oil emulsion vaccines in broiler breeder chickens. I. Newcastle disease virus and infectious bursal disease virus bivalent vaccines. II. Trivalent vaccines in breeders not previously vaccinated with live Newcastle disease, infectious bursal disease, and tenosynovitis vaccines. III. Trivalent Newcastle disease, infectious bursal disease, and arthritis/tenosynovitis viruses vaccine in primed breeders. Poultry Science 62(10): 1978-1997, 1983

Infectious bursal disease virus structural protein VP2 expressed by a fowlpox virus recombinant confers protection against disease in chickens. Archives of Virology 131(3-4): 277-292, 1993

Protection of chickens against very virulent infectious bursal disease virus and Mareks disease virus with a recombinant MDV expressing IBDV VP2. Virology 257(2): 352-362, 1999

Protection of chickens against very virulent infectious bursal disease virus with recombinant Mareks Disease virus expressing IBDV VP2 antigen. Proceedings of Western Poultry Disease Conference: 8) 49, 1999

DNA-mediated vaccination conferring protection against infectious bursal disease in broiler chickens in the presence of maternal antibody. Vaccine 28(23): 3936-3943, 2010

Evaluation of safety and efficacy of vaccination of chickens with live recombinant fowlpox expressing infectious bursal disease virus antigens. International symposium on infectious bursal disease and chicken infectious anaemia, Rauischholzhausen, Germany, 21-24 June, 1994: 336-339, 1994

Partial protection against infectious bursal disease virus through DNA-mediated vaccination with the VP2 capsid protein and chicken IL-2 genes. Vaccine 22(9-10): 1249-1259, 2004