+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches



Identification and interaction of multiple genes resulting in DDT resistance in the 91-R strain of Drosophila melanogaster by RNAi approaches



Pesticide Biochemistry and Physiology 151: 90-99



4,4'-dichlorodiphenyltrichloroethane (DDT) has been re-recommended by the World Health Organization for malaria mosquito control. Previous DDT use has resulted in resistance, and with continued use resistance will likely increase in terms of level and extent. Drosophila melanogaster is a model dipteran with a well annotated genome allowing both forward and reverse genetic manipulation, numerous studies done on insecticide resistance mechanisms, and is related to malaria mosquitoes allowing for extrapolation. The 91-R strain of D. melanogaster is highly resistant to DDT (>1500-fold) and recently, reduced penetration, increased detoxification, and direct excretion have been identified as resistance mechanisms. Their interactions, however, remain unclear. Use of Gal4/UAS-RNAi transgenic lines of D. melanogaster allowed for the targeted knockdown of genes putatively involved in DDT resistance and has identified the role of several cuticular proteins (Cyp4g1 and Lcp1), cytochrome P450 monooxygenases (Cyp6g1 and Cyp12d1), and ATP binding cassette transporters (Mdr50, Mdr65, and Mrp1) involved in decreased sensitivity to DDT. These above findings have been further validated in 91-R flies using a nanoparticle-enhanced RNAi strategy, directly implication these genes in DDT resistance in 91-R flies.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 066446800

Download citation: RISBibTeXText

PMID: 30704719

DOI: 10.1016/j.pestbp.2018.03.003


Related references

RNAi validation of resistance genes and their interactions in the highly DDT-resistant 91-R strain of Drosophila melanogaster. Pesticide Biochemistry and Physiology 121: 107-115, 2016

Studies in homocotic genes antennopedix (Apx) and nasobemia (Ns) of Drosophila melanogaster. 2. Interaction of genes Apx and Ns with polycomb, multiple sex cb, aristapedia, thread. Genetika, 89: 67-74, 1972

Studies on homoeotic genes Antennopedix (Apx) and Nasobemia (Ns) of Drosophila melanogaster. II. Interaction of genes Apx and Ns with Polycomb, Multiple sex comb, Aristapedia, Thread. Genetika 8 (9) 67-74, 1972

RNAi screening of Drosophila (Sophophora) melanogaster S2 cells for ricin sensitivity and resistance. Journal of Biomolecular Screening 16(4): 436-442, 2011

An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster. Zoological Science 33(6): 583-591, 2017

Identification of novel modulators of mitochondrial function by a genome-wide RNAi screen in Drosophila melanogaster. Genome Research 18(1): 123-136, 2007

Studies on homoeotic genes antennopedix and nosobemia of drosophila melanogaster part 2 interaction of genes antennopedix and nosobemia with polycomb multiple sex comb aristapedia thread. Genetika 8(9): 67-74, 1972

A study of the homeotic genes antennopedix and nasobemia in Drosophila melanogaster. Communication II. Interaction of the homeotic antennopedix and nasobemia genes with the polycomb, multiple sex comb, aristapedia, and thread genes. Soviet Genetics 8(9): 1126-1131, 1974

A study of the homeotic genes Antennopedix and Nasobemia in Drosophila melanogaster. II. Interaction of the homeotic Antennopedix and Nasobemia genes with the Polycomb, Multiple sex comb, aristapedia, and thread genes. Soviet genetics: transl t 1974, 8 (9) 1126-1131, 1972

Intersexuality resulting from the interaction of sex-specific lethal mutations in Drosophila melanogaster. Developmental Biology 94(1): 153-162, 1982

Sonless, a sex-ratio anomaly in Drosophila melanogaster resulting from a gene-cytoplasm interaction. Genetics 65(4): 619-625, 1970

Wing and leg phenotypes resulting from the interaction of the cubitus interruptus mutants ci w and ci d in drosophila melanogaster. Genetics 83(3 PART 1): S7, 1976

Sonless, a sex-ration anomaly in Drosophila melanogaster resulting from a gene-cytoplasm interaction. Genetics 65 (4) 619-625, 1970

Identification of elite potato genotypes possessing multiple disease resistance genes through molecular approaches. Scientia Horticulturae 179: 204-211, 2014

Leg and wing phenotypes resulting from the interaction of the mutants Cubitus interruptus-Wallace (ciW) and--dominant (ciD) in Drosophila melanogaster. Journal of the Tennessee Academy of Science 59(59): 57-61, 1984